ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSELHALLTF PERPISQSYY VPKLQHFLKS GIPATYTLEQ VAAFEHEEKN RNGDKEFRES 

        70         80         90        100        110        120 
TDDNKTSNTT PLHVLARSLP LDIKDEELQV VMDMMNILFE YGAGWNFIDY EDKTVGDLFL 

       130        140        150        160        170        180 
ERNQSRESPL YRRLVEAGVS AELLLRKLNG GDVEFLDTDE LIGIEPEESV QTAVDGQKEE 

       190        200        210        220        230        240 
SVGSDDDATA ANQQVYLKTE LEYKDDALIT KENKDGVMMD WETKIMELAS ETLFPDPEAT 

       250        260        270        280        290        300 
NSATILNIGF GMGIIDTFIQ ARKPYRHYIC EAHPDVLAKM KMDGWYEKDN VVILEGRWQD 

       310        320        330        340        350        360 
TLNNLLDKGE VFFDGIYYDT FSEHYQDILD LYDVVVGLIK PEGVFSFFNG LGADRSLCYD 

       370        380        390        400        410 
VYKEIVEIDV ATYGMKCDYT RYSLDEQLPD WNDVKRSYFN CNYYYHPRIT FA 

References and documentation are available.

Number of amino acids: 412

Molecular weight: 47469.14

Theoretical pI: 4.44

Amino acid composition: 
Ala (A)  22	  5.3%
Arg (R)  16	  3.9%
Asn (N)  19	  4.6%
Asp (D)  39	  9.5%
Cys (C)   4	  1.0%
Gln (Q)  13	  3.2%
Glu (E)  39	  9.5%
Gly (G)  24	  5.8%
His (H)   8	  1.9%
Ile (I)  22	  5.3%
Leu (L)  40	  9.7%
Lys (K)  23	  5.6%
Met (M)  11	  2.7%
Phe (F)  19	  4.6%
Pro (P)  15	  3.6%
Ser (S)  20	  4.9%
Thr (T)  23	  5.6%
Trp (W)   5	  1.2%
Tyr (Y)  24	  5.8%
Val (V)  26	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      2126
Hydrogen    H	      3232
Nitrogen    N	       536
Oxygen      O	       668
Sulfur      S	        15

Formula: C2126H3232N536O668S15
Total number of atoms: 6577

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63510
Abs 0.1% (=1 g/l)   1.338, assuming all pairs of Cys residues form cystines


Ext. coefficient    63260
Abs 0.1% (=1 g/l)   1.333, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.12
This classifies the protein as unstable.



Aliphatic index: 82.33

Grand average of hydropathicity (GRAVY): -0.461