ProtParam
User-provided sequence:
10 20 30 40 50 60
MSELQSQPKK IAVVTGASGG IGYEVTKELA RNGYLVYACA RRLEPMAQLA IQFGNDSIKP
70 80 90 100 110 120
YKLDISKPEE IVTFSGFLRA NLPDGKLDLL YNNAGQSCTF PALDATDAAV EQCFKVNVFG
130 140 150 160 170 180
HINMCRELSE FLIKAKGTIV FTGSLAGVVS FPFGSIYSAS KAAIHQYARG LHLEMKPFNV
190 200 210 220 230 240
RVINAITGGV ATDIADKRPL PETSIYNFPE GREAFNSRKT MAKDNKPMPA DAYAKQLVKD
250 260 270 280 290
ILSTSDPVDV YRGTFANIMR FVMIFVPYWL LEKGLSKKFK LDKVNNALKS KQKNKDD
References and
documentation are available.
Number of amino acids: 297
Molecular weight: 32813.94
Theoretical pI: 9.23
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1479
Hydrogen H 2340
Nitrogen N 392
Oxygen O 426
Sulfur S 12
Formula: C
1479H
2340N
392O
426S
12
Total number of atoms: 4649
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22140
Abs 0.1% (=1 g/l) 0.675, assuming all pairs of Cys residues form cystines
Ext. coefficient 21890
Abs 0.1% (=1 g/l) 0.667, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.61
This classifies the protein as stable.
Aliphatic index: 85.76
Grand average of hydropathicity (GRAVY): -0.161