ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSENNGNPLD LSSLRNKISS KLRDNNSKKA KKTHKGKDVK ASSNSKKVNE DIRREALALG 

        70         80         90        100        110        120 
ASEEDLKLIQ GLSDDDDAKS EQEFDAVADE DADDKGFKND LQNFMKNVGF DQHKLEDVDD 

       130        140        150        160        170        180 
DDIEEESTSS KESKIPAQEK EHAQSNIASS TIEKTSQESI DNGSEQEENT VEEANLSSDQ 

       190        200        210        220        230        240 
EPESESAEKE KKEEKDGGLI TQTTIISSDK LIIPYDKPWY EIPLDPQVGQ NDDVEELSKE 

       250        260        270        280        290        300 
QIEKLFERGK QTLEADNQTY YEEFTKDSSQ AKFMSQILSD GTLNDKISAV TLLIQDSPLH 

       310        320        330        340        350        360 
NTKSLETLVS YCGKKSRNSA LQSLNALKDL FLNGLLPNRK LRYFKNQPGL SMMLNKKTLA 

       370        380        390        400        410        420 
IFYFEDYLKK LFFRVLEVLE VLSHDPIIHV RLQILNHVFD LLTNQPEQEF NLLRLGVNKI 

       430        440        450        460        470        480 
GDIDSKVSSK ASYLLLKLEQ AHPNMKSIVI DAIVDIALRP NADYHTTYYS VITLNQTILK 

       490        500        510        520        530        540 
RSEDSVANKL VKTYFTLFEK FLINTDKDNT NGVVKSNSKS YEEKRKKNFK KGKHGGKSVK 

       550        560        570        580        590        600 
IEKTENEVLD EKNSKLFSAL LTGINRAFPF AQIPASVYEV HMETLFKITH SSNFNTSIQA 

       610        620        630        640        650        660 
LVLINQVTVK AKLNSDRYYR TLYESLFDPR LVNSSKQGIY LNLLYKSLKQ DALNVERVEA 

       670        680        690        700        710        720 
FVKRILQVCS HWLNVGTITG FFFLLIQLAK TVPQIKNLLT NTPVDYEYES DAEEEQGDKD 

       730        740        750        760        770        780 
IKRKEYDGRK RDPKFANAEK SSLWEINNFI NHFHPTVKTY ANAYVTGETE QIAKPDLGLF 

       790        800        810        820        830        840 
TLSHFLDRFV YRSAKQTNTA RGTSIMQPLF SGSRVNDSVL VKASDIMHDQ GPVNTEDWLT 

       850        860        870        880        890        900 
KKVEDIKPED KFFYQYFTTK KTADGKGKKS NKASNFDSDD EMDENEIWSA LVKSRPDVED 

       910        920        930        940        950        960 
DSDDSELDFA EDDFSDSTSD DEPKLDAIDD EDAKSEGSQE SDQEEGLDED IFYSFDGEQD 

       970        980        990       1000       1010       1020 
NSDKKRSFAE SSEEDESSEE EKEEEENKEV SAKRAKKKQR KNMLKSLPVF ASADDYAQYL 


DQDSD 

References and documentation are available.

Number of amino acids: 1025

Molecular weight: 116676.54

Theoretical pI: 4.89

Amino acid composition: 
Ala (A)  56	  5.5%
Arg (R)  31	  3.0%
Asn (N)  65	  6.3%
Asp (D)  96	  9.4%
Cys (C)   2	  0.2%
Gln (Q)  45	  4.4%
Glu (E)  97	  9.5%
Gly (G)  37	  3.6%
His (H)  17	  1.7%
Ile (I)  52	  5.1%
Leu (L)  96	  9.4%
Lys (K) 108	 10.5%
Met (M)  11	  1.1%
Phe (F)  46	  4.5%
Pro (P)  28	  2.7%
Ser (S) 100	  9.8%
Thr (T)  51	  5.0%
Trp (W)   5	  0.5%
Tyr (Y)  31	  3.0%
Val (V)  51	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 193
Total number of positively charged residues (Arg + Lys): 139

Atomic composition:

Carbon      C	      5128
Hydrogen    H	      8081
Nitrogen    N	      1375
Oxygen      O	      1704
Sulfur      S	        13

Formula: C5128H8081N1375O1704S13
Total number of atoms: 16301

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73815
Abs 0.1% (=1 g/l)   0.633, assuming all pairs of Cys residues form cystines


Ext. coefficient    73690
Abs 0.1% (=1 g/l)   0.632, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.30
This classifies the protein as unstable.



Aliphatic index: 76.20

Grand average of hydropathicity (GRAVY): -0.807