ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSENQKEVSP PQAISVKSEA SSSIFSKPIS TSSPAGLAAA QRVTPGKLST LLLEMGPLAI 

        70         80         90        100        110        120 
RHITQTLCLD IPCFKDLSSS KQRRLIMSAM ESGDKEKSVV FEKIGWGQWS AKRVDPANFD 

       130        140        150        160        170        180 
KELEATNFAN AKVKDLISQE SQRRKSNNSN SNSGGKVEMP MKVEHNITNI DGATTPPTAV 

       190        200        210        220        230        240 
ASTTIPVNIK RSKSPLAAAN VVYIDENALA SEDEDEEFDE DDHHLHYQNK SRNSSNNFGK 

       250        260        270        280        290        300 
SSNGDPYSFG RRRSQVVFAD STPENIEHEI IAQKIRPLLR NRRRSSIKPH TPFISKLNTH 

       310        320        330        340        350        360 
QDSSYLSPNT TSTTTPSNNN SNSNQAKIDL EKLTATSEPT SRRASRLSVS KESSIRSTLF 

       370        380        390        400        410        420 
PNKNYLIVTT NPNSKATSVS TSPKLEEQMN VSSNPIVLSD KEKHRVASQH LNGESSPQLV 

       430        440        450        460        470        480 
PHSHHQPHSD TDEEDWESIG AASLRNNSLA PNIDSVASST NGVVSPKPTN PSFTNSQNGD 

       490        500        510 
IEPPLQHDQQ KHEQQPRNGE DNSAAFLLMS LKS 

References and documentation are available.

Number of amino acids: 513

Molecular weight: 56264.16

Theoretical pI: 6.99

Amino acid composition: 
Ala (A)  34	  6.6%
Arg (R)  24	  4.7%
Asn (N)  43	  8.4%
Asp (D)  25	  4.9%
Cys (C)   2	  0.4%
Gln (Q)  23	  4.5%
Glu (E)  34	  6.6%
Gly (G)  17	  3.3%
His (H)  16	  3.1%
Ile (I)  27	  5.3%
Leu (L)  34	  6.6%
Lys (K)  34	  6.6%
Met (M)   8	  1.6%
Phe (F)  13	  2.5%
Pro (P)  35	  6.8%
Ser (S)  79	 15.4%
Thr (T)  32	  6.2%
Trp (W)   3	  0.6%
Tyr (Y)   5	  1.0%
Val (V)  25	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2409
Hydrogen    H	      3870
Nitrogen    N	       720
Oxygen      O	       814
Sulfur      S	        10

Formula: C2409H3870N720O814S10
Total number of atoms: 7823

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24075
Abs 0.1% (=1 g/l)   0.428, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.426, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.24
This classifies the protein as unstable.



Aliphatic index: 67.13

Grand average of hydropathicity (GRAVY): -0.806