ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEPSKEELL ARIAQLELEN EQLKQQNGKK SQHEQFNKID DNFSLDEYKR YGRQMIVPQF
70 80 90 100 110 120
GSLESQIKLK NSKVLVVGAG GLGSPALLYL SSAGIGKIGI IDPDTVDTSN LHRQVIHNTE
130 140 150 160 170 180
MVGEFKCISA QNYINKLNPH VVVEVYPTAL NNDNAFGIVS QYDLVLDCTD HPAVRYLIND
190 200 210 220 230 240
VCVLLGKTIV SGSGLKSDGQ LTVLNFANSG PCYRCFYPQP PSPDSVTSCS DGGVIGPAIG
250 260 270 280 290 300
LVGVAMAVET IKIITGYYTK DNFVPFLASY SAYPQQQLRV FKMRKRQKDC AVCGENPQIS
310 320 330 340 350 360
QRMIEDGTIN YKTFCGRATF DPIDDKFRVS PKDYDSVVQN KKKHILIDVR PREQFQITHL
370 380 390 400 410 420
PNAINVQWDP TFRKADAIEQ YLPDDSTKDD EIYVVCRFGN DSQLAAKKLI GMGYPNVRDI
430
IGGLDKWSDD VDSKIPKY
References and
documentation are available.
Number of amino acids: 438
Molecular weight: 48866.60
Theoretical pI: 6.03
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2171
Hydrogen H 3421
Nitrogen N 587
Oxygen O 661
Sulfur S 17
Formula: C
2171H
3421N
587O
661S
17
Total number of atoms: 6857
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39935
Abs 0.1% (=1 g/l) 0.817, assuming all pairs of Cys residues form cystines
Ext. coefficient 39310
Abs 0.1% (=1 g/l) 0.804, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.62
This classifies the protein as stable.
Aliphatic index: 86.96
Grand average of hydropathicity (GRAVY): -0.351