ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEQHSVAVG KAANEHETAP RRNVRVKKRP LIRPLNSSAS TLYEFALECF NKGGKRDGMA 

        70         80         90        100        110        120 
WRDVIEIHET KKTIVRKVDG KDKSIEKTWL YYEMSPYKMM TYQELIWVMH DMGRGLAKIG 

       130        140        150        160        170        180 
IKPNGEHKFH IFASTSHKWM KIFLGCISQG IPVVTAYDTL GESGLIHSMV ETESAAIFTD 

       190        200        210        220        230        240 
NQLLAKMIVP LQSAKDIKFL IHNEPIDPND RRQNGKLYKA AKDAINKIRE VRPDIKIYSF 

       250        260        270        280        290        300 
EEVVKIGKKS KDEVKLHPPE PKDLACIMYT SGSISAPKGV VLTHYNIVSG IAGVGHNVFG 

       310        320        330        340        350        360 
WIGSTDRVLS FLPLAHIFEL VFEFEAFYWN GILGYGSVKT LTNTSTRNCK GDLVEFKPTI 

       370        380        390        400        410        420 
MIGVAAVWET VRKAILEKIS DLTPVLQKIF WSAYSMKEKS VPCTGFLSRM VFKKVRQATG 

       430        440        450        460        470        480 
GHLKYIMNGG SAISIDAQKF FSIVLCPMII GYGLTETVAN ACVLEPDHFE YGIVGDLVGS 

       490        500        510        520        530        540 
VTAKLVDVKD LGYYAKNNQG ELLLKGAPVC SEYYKNPIET AVSFTYDGWF RTGDIVEWTP 

       550        560        570        580        590        600 
KGQLKIIDRR KNLVKTLNGE YIALEKLESV YRSNSYVKNI CVYADESRVK PVGIVVPNPG 

       610        620        630        640        650        660 
PLSKFAVKLR IMKKGEDIEN YIHDKALRNA VFKEMIATAK SQGLVGIELL CGIVFFDEEW 

       670        680        690 
TPENGFVTSA QKLKRREILA AVKSEVERVY KENS 

References and documentation are available.

Number of amino acids: 694

Molecular weight: 77946.54

Theoretical pI: 9.10

Amino acid composition: 
Ala (A)  45	  6.5%
Arg (R)  28	  4.0%
Asn (N)  29	  4.2%
Asp (D)  29	  4.2%
Cys (C)  10	  1.4%
Gln (Q)  13	  1.9%
Glu (E)  49	  7.1%
Gly (G)  51	  7.3%
His (H)  16	  2.3%
Ile (I)  56	  8.1%
Leu (L)  52	  7.5%
Lys (K)  67	  9.7%
Met (M)  18	  2.6%
Phe (F)  28	  4.0%
Pro (P)  28	  4.0%
Ser (S)  44	  6.3%
Thr (T)  34	  4.9%
Trp (W)  11	  1.6%
Tyr (Y)  27	  3.9%
Val (V)  59	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      3532
Hydrogen    H	      5588
Nitrogen    N	       930
Oxygen      O	       998
Sulfur      S	        28

Formula: C3532H5588N930O998S28
Total number of atoms: 11076

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   101355
Abs 0.1% (=1 g/l)   1.300, assuming all pairs of Cys residues form cystines


Ext. coefficient   100730
Abs 0.1% (=1 g/l)   1.292, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.64
This classifies the protein as stable.



Aliphatic index: 91.83

Grand average of hydropathicity (GRAVY): -0.161