ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEQNSVVNA EKGDGEISSN VETASSVNPS VKPQNAIKEE AKETNGEDQK CKGPENAGST 

        70         80         90        100        110        120 
AETKETSNDA TNGMKTPEET EDTNDKRHDD EGEDGDEDED EDEDEDEDNG DEDDEDVDSS 

       130        140        150        160        170        180 
SSETSSEDGE DSESVSGEST ESSSGEDEES DESDGNTSNS SSGDESGSEE EEEEEEEEEE 

       190        200        210        220        230        240 
EENAGEPSIA HQDSVPTNDS TAPRSTHTRN ISLSSNGSNT NSTIILVKTT LETILNDKDI 

       250        260        270        280        290        300 
KKNSNAQKAI ERTLQKFKEF DPQTTNNPHY VDSILVFEAL RASCRTKSSK VQSLALDCLS 

       310        320        330        340        350        360 
KLFSFRSLDE TLLVNPPDSL ASNDQRQDAA DGITPPPKQK IIDAAIDTIS DCFQGEGTDD 

       370        380        390        400        410        420 
RVELQIVRAL SSCILEEDSS SLCHGASLLK AIRTIYNVLL FCLNPSNQGI AQATLTQIIS 

       430        440        450        460        470        480 
SVYDKIDLKQ STSSAVSLST KNHQQQSAIE LSEASENAET PAPLTLENMD KLNDDEERLM 

       490        500        510        520        530        540 
DAQQPDSIAI TNQDLAVKDA FLVFRVMAKI CAKPLETELD MRSHAVRSKL LSLHIIYSII 

       550        560        570        580        590        600 
KDHIDVFLSH NIFLPGKERV CFIDSIRQYL RLVLSRNAAS PLAPVFEVTL EIMWLLIANL 

       610        620        630        640        650        660 
RADFVKEIPV FLTEIYFPIS ELTTSTSQQK RYFLSVIQRI CNDPRTLVEF YLNYDCNPGM 

       670        680        690        700        710        720 
PNVMEITVDY LTRLALTRVE ITQTQRSYYD EQISKSLSTY NFSQLPLLTS SNLSSSPDVG 

       730        740        750        760        770        780 
QVNLLFPLDF ALKMVSLNCI VSVLRSLSSW AHKALNPNTH TANKVLLNTT SSARQESRSS 

       790        800        810        820        830        840 
LSNDVRSSIM TSNDDFKPTY EDEESRSLSS QNIDADDPTQ FENLKLRKTA LSECIAIFNN 

       850        860        870        880        890        900 
KPKKAIPVLI KKGFLKDDSP ISIAKWLLET EGLDMAAVGD YLGEGDDKNI AIMHAFVDEF 

       910        920        930        940        950        960 
DFTGMSIVDA LRSFLQSFRL PGEGQKIDRF MLKFAERFVD QNPGVFSKAD TAYVLSYSLI 

       970        980        990       1000       1010       1020 
MLNTDLHSSQ IKNKMSLQEF LENNEGIDNG RDLPRDFLEG LFNEIANNEI KLISEQHQAM 

      1030       1040       1050       1060       1070       1080 
LSGDTNLVQQ PAICFQLFNS RDLTREAYNQ VSKEISSKTE LVFKNLNKNK GGPDVYYAAS 

      1090       1100       1110       1120       1130       1140 
HVEHVKSIFE TLWMSFLAAL TPPFKDYDDI DTTNKCLEGL KISIKIASTF RINDARTSFV 

      1150       1160       1170       1180       1190       1200 
GALVQFCNLQ NLEEIKVKNV NAMVILLEVA LSEGNYLEGS WKDILLVVSQ MERLQLISKG 

      1210       1220       1230       1240       1250       1260 
IDRDTVPDVA QARVANPRVS YESSRSNNTS FFDVWGKKAT PTELAQEKHH NQTLSPEISK 

      1270       1280       1290       1300       1310       1320 
FISSSELVVL MDNIFTKSSE LSGNAIVDFI KALTAVSLEE IESSENASTP RMFSLQKMVD 

      1330       1340       1350       1360       1370       1380 
VCYYNMDRIK LEWTPLWAVM GKAFNKIATN SNLAVVFFAI DSLRQLSMRF LDIEELSGFE 

      1390       1400       1410       1420       1430       1440 
FQHDFLKPFE YTVQNSGNTE VQEMIIECFR NFILTKSESI KSGWKPILES LQYTARSSTE 

      1450       1460       1470       1480       1490       1500 
SIVLKTQLLV SNDIVTNHFE NVFSQEDAFS ELVGVFREIT KNKRFQKLSL HALESLRKMT 

      1510       1520       1530       1540       1550       1560 
QNVADICFYN ENKTEEERKH NDALLRGKDI FQDVWFPMLF CFNDTIMTAE DLEVRSRALN 

      1570       1580       1590       1600       1610       1620 
YMFDALVAYG GKFNDDFWEK ICKKLLFPIF GVLSKHWEVN QFNSHDDLSV WLSTTLIQAL 

      1630       1640       1650       1660       1670       1680 
RNLIALFTHY FESLNRMLDG FLGLLVSCIC QENDTIARIG RSCLQQLILQ NVSKFNEYHW 

      1690       1700       1710       1720       1730       1740 
NQIGDVFDKL FDLTTANELF DYDPLQQGRK SSVSHHQTTN DTSQHSDDDS NDRRENDSNI 

      1750       1760       1770       1780       1790       1800 
SETVERAHQE ESSEDVGGDM VETLNGQTKL NNGNSVPTVK DELNPKPASL SIPKKTKHMK 

      1810       1820       1830       1840       1850       1860 
RNESNEDIRR RINIKNSIVV KCVLQLLMIE LLNELFENED FAHCIPYKEA IRITRLLEKS 

      1870       1880       1890       1900       1910       1920 
YEFSRDFNED YGLRTRLVEA RVVDKIPNLL KQETSAAAVL LDIMFQLYLN DDEKKADLIT 

      1930       1940       1950       1960       1970       1980 
RLITICIQVV EGYVSLDDRT MERSINAWRS VIVEILQGYY EFDDEDFRLY CPAMYALVIQ 

      1990       2000 
ILDKSVPTEL RHAIKQFLSR VGELYLSTD 

References and documentation are available.

Number of amino acids: 2009

Molecular weight: 226848.14

Theoretical pI: 4.68

Amino acid composition: 
Ala (A) 114	  5.7%
Arg (R)  85	  4.2%
Asn (N) 131	  6.5%
Asp (D) 152	  7.6%
Cys (C)  28	  1.4%
Gln (Q)  84	  4.2%
Glu (E) 175	  8.7%
Gly (G)  69	  3.4%
His (H)  34	  1.7%
Ile (I) 129	  6.4%
Leu (L) 204	 10.2%
Lys (K) 116	  5.8%
Met (M)  39	  1.9%
Phe (F)  93	  4.6%
Pro (P)  62	  3.1%
Ser (S) 205	 10.2%
Thr (T) 116	  5.8%
Trp (W)  15	  0.7%
Tyr (Y)  43	  2.1%
Val (V) 115	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 327
Total number of positively charged residues (Arg + Lys): 201

Atomic composition:

Carbon      C	      9947
Hydrogen    H	     15706
Nitrogen    N	      2678
Oxygen      O	      3243
Sulfur      S	        67

Formula: C9947H15706N2678O3243S67
Total number of atoms: 31641

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   148320
Abs 0.1% (=1 g/l)   0.654, assuming all pairs of Cys residues form cystines


Ext. coefficient   146570
Abs 0.1% (=1 g/l)   0.646, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.60
This classifies the protein as unstable.



Aliphatic index: 86.92

Grand average of hydropathicity (GRAVY): -0.415