ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEQSQLDDS TIDKLIPQIF NEMRSNLNNT TNKFPKSTGG GASDNISANS NSIRSFNSIT 

        70         80         90        100        110        120 
TQSLLKESES LDKITAMIKN VTAALKNNLP VYVNQVHEVC KSTNSILDSW INIHSQAGYI 

       130        140        150        160        170        180 
HKLMSDQTYL KLINDRLHNE NVNTNDEDGS TLHNVIALKK KEILDLRQKL ENRKGEKDAA 

       190        200        210        220        230        240 
PAKPPNQGLN PRYGVQSGRR PVPSAGISNN GRVRKTHVPA SKRPSGIPRV TNRWTKPTAS 


SSRKMFR 

References and documentation are available.

Number of amino acids: 247

Molecular weight: 27472.96

Theoretical pI: 9.96

Amino acid composition: 
Ala (A)  13	  5.3%
Arg (R)  15	  6.1%
Asn (N)  27	 10.9%
Asp (D)  12	  4.9%
Cys (C)   1	  0.4%
Gln (Q)  10	  4.0%
Glu (E)  10	  4.0%
Gly (G)  12	  4.9%
His (H)   6	  2.4%
Ile (I)  17	  6.9%
Leu (L)  19	  7.7%
Lys (K)  21	  8.5%
Met (M)   5	  2.0%
Phe (F)   4	  1.6%
Pro (P)  13	  5.3%
Ser (S)  28	 11.3%
Thr (T)  16	  6.5%
Trp (W)   2	  0.8%
Tyr (Y)   4	  1.6%
Val (V)  12	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1182
Hydrogen    H	      1938
Nitrogen    N	       364
Oxygen      O	       377
Sulfur      S	         6

Formula: C1182H1938N364O377S6
Total number of atoms: 3867

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.617, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.617, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.94
This classifies the protein as unstable.



Aliphatic index: 76.19

Grand average of hydropathicity (GRAVY): -0.792