ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSERDFNTQS PKFKDENANS VISQSDFLGK SLKNNNDDYS DFRGSYLNDK SSFQTPLRNG 

        70         80         90        100        110        120 
SYQPKGSLEF TPLLQSSNKN SDKYNGSLGD KGSFDPNSYG LSAISKQATQ EALSISQGND 

       130        140        150        160        170        180 
SYDVSKLTDL SKNSEIDHTD GELPANAALT LREQEKVLEK VSRENFGLRI KIVCLEKRLE 

       190        200        210        220        230        240 
SMAPEQIKEA VKDNVELHAE RANLQLQLKR TESLLQKSED KNFKLEEKVD YLSKVNDVEQ 

       250        260        270        280        290        300 
SQNVKVFTER IRFLENALEK VQREKDSLST EMEEDKSNKE VDYEYEIRQL QNRLDELSEE 

       310        320        330        340        350        360 
LDVAQDLLTE KEDEIATLKR QIEEKENSSS AFENEENSSY VHLQEDYAIL QAKCDEFADR 

       370        380        390        400        410        420 
IQVLTADLEK EKENQIMHES EASIGLTDSM QVHTLQEQLH KANEEIEFLH DQISRMNEEG 

       430        440        450        460        470        480 
KNFEDIMLQF RSLEEERDVL ESKLQTLEDD NNSLRLMTSS LGNQIESLRT QNREIDEEKN 

       490        500        510        520        530        540 
HLRLLASKNS DKALAETNIR LQEVTKELET LRMKNSNDLN EIHDLREENE GLTLKIDSIT 

       550        560        570        580        590        600 
KEKDRLINEL EQRIKSYEVN VSELNGTIDE YRNKLKDKEE TYNEVMNAFQ YKDNDLRRFH 

       610        620        630        640        650        660 
ESINKLQDRE KELTSNLEKK NLVISSLRET VAMLEKERES IKKYLSGNAK DLDNTNLMEI 

       670        680        690        700        710        720 
LNDKISVLQR QLTDVKDELD VSEEEREEAI VAGQKLSASF ELMSNEKQAL ELKYSSLKNE 

       730        740        750        760        770        780 
LINAQNLLDR REEELSELSK KLFEERKIRS GSNDDIEKNK EINVLNSELA DKLAQIRHLE 

       790        800        810        820        830        840 
SDKMELDKLV HHLNRGIEEA NIEENAVKKR LCLLMGCDYS SVSILQIVSQ IEHFVNQQIQ 

       850        860        870        880        890        900 
TIRSLKQELR HDFVQFSGKK EQELSRSFEK FGLGTETKHD ILAQRNRNVS EKMNDLENAA 

       910        920        930        940        950        960 
QKFFSSPDRK NGYLYPSEHT SKIEYLEKTI EDLKLALQDE LKNRNLLMDD ISSYNKQTTK 

       970        980        990       1000       1010       1020 
LQEKIKWLER ERSILIDELE SYRSNQFNYQ NNLVQDKNEL EERLKEIQKE LEVYNNHFMK 

      1030       1040       1050       1060       1070       1080 
QAELMTSNVT DESQLMLKTL REALQSKTNN IDHLSTILER NRKEYKSLLD DYNQLRARYK 

      1090       1100       1110       1120       1130       1140 
NLQSNTPQST QSGQYESEIK GLSKLTKYLQ SKCRREHSLR LDLAFSKKFI LMQLTGYETC 

      1150       1160       1170       1180       1190       1200 
NKINLRMLQK IGISPDPDLS KKHIKLKSLI IVVCSIERMK RMKNEWLKQA QLKQSLQRAA 


AKAKTANY 

References and documentation are available.

Number of amino acids: 1208

Molecular weight: 140763.69

Theoretical pI: 5.22

Amino acid composition: 
Ala (A)  50	  4.1%
Arg (R)  66	  5.5%
Asn (N)  94	  7.8%
Asp (D)  77	  6.4%
Cys (C)   7	  0.6%
Gln (Q)  74	  6.1%
Glu (E) 152	 12.6%
Gly (G)  29	  2.4%
His (H)  21	  1.7%
Ile (I)  64	  5.3%
Leu (L) 154	 12.7%
Lys (K) 118	  9.8%
Met (M)  24	  2.0%
Phe (F)  31	  2.6%
Pro (P)  12	  1.0%
Ser (S) 112	  9.3%
Thr (T)  47	  3.9%
Trp (W)   2	  0.2%
Tyr (Y)  32	  2.6%
Val (V)  42	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 229
Total number of positively charged residues (Arg + Lys): 184

Atomic composition:

Carbon      C	      6084
Hydrogen    H	      9886
Nitrogen    N	      1736
Oxygen      O	      2026
Sulfur      S	        31

Formula: C6084H9886N1736O2026S31
Total number of atoms: 19763

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59055
Abs 0.1% (=1 g/l)   0.420, assuming all pairs of Cys residues form cystines


Ext. coefficient    58680
Abs 0.1% (=1 g/l)   0.417, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.64
This classifies the protein as unstable.



Aliphatic index: 84.60

Grand average of hydropathicity (GRAVY): -0.928