ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSERKAINKY YPPDYNPLEA EKLSRKMAKK LKTMNKSHAS IRLMTPFSMR CLECNEYIPK 

        70         80         90        100        110        120 
SRKFNGKKEL LKEKYLDSIK IYRLTISCPR CANSIAFRTD PGNSDYVMEV GGVRNYVPQK 

       130        140        150        160        170        180 
PNDDLNAKTA VESIDETLQR LVREKEMEQN EKMGIKEQAD DKMDLLEKRL AKIQQEQEDD 

       190        200        210        220        230        240 
EELENLRKKN LEMSQRAEMI NRSKHAQQEK AVTTDDLDNL VDQVFDNHRQ RTNKPGNNND 

       250        260        270 
EKRTPLFNPT STKGKIQKKS SVRTNPLGIV IKRGKSLK 

References and documentation are available.

Number of amino acids: 278

Molecular weight: 32311.95

Theoretical pI: 9.41

Amino acid composition: 
Ala (A)  13	  4.7%
Arg (R)  20	  7.2%
Asn (N)  22	  7.9%
Asp (D)  18	  6.5%
Cys (C)   4	  1.4%
Gln (Q)  13	  4.7%
Glu (E)  25	  9.0%
Gly (G)   9	  3.2%
His (H)   3	  1.1%
Ile (I)  14	  5.0%
Leu (L)  23	  8.3%
Lys (K)  36	 12.9%
Met (M)  11	  4.0%
Phe (F)   5	  1.8%
Pro (P)  13	  4.7%
Ser (S)  17	  6.1%
Thr (T)  13	  4.7%
Trp (W)   0	  0.0%
Tyr (Y)   8	  2.9%
Val (V)  11	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      1390
Hydrogen    H	      2297
Nitrogen    N	       415
Oxygen      O	       438
Sulfur      S	        15

Formula: C1390H2297N415O438S15
Total number of atoms: 4555

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12170
Abs 0.1% (=1 g/l)   0.377, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.369, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.14
This classifies the protein as stable.



Aliphatic index: 68.06

Grand average of hydropathicity (GRAVY): -1.099