ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL KKKTLQIAET 

        70         80         90        100        110        120 
TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP SIDCVLCCIY EVPRDISTRI 

       130        140        150        160        170        180 
GLCIINCNTG QMYLSDFMDS QIYIRVVHKL QIYQPTEILI PSSSLAPTVS KLATMIKFNV 

       190        200        210        220        230        240 
AETVKIEEGS RKCFNSQDGL AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI 

       250        260        270        280        290        300 
ISKSSRNLNA FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM 

       310        320        330        340        350        360 
GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD KLFSRLLCIN 

       370        380        390        400        410        420 
HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI SETKKIFNND AIMEIEKLIN 

       430        440        450        460        470        480 
SCINEDCVWA SSAIQLLNQR SYAVKSDSNG LLDVSRQIYK EVKEEFFREV EDLTAKNKIN 

       490        500        510        520        530        540 
LDHNYDSARG FYLRIKRQEF TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME 

       550        560        570        580        590        600 
EILLLSEETV DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI 

       610        620        630        640        650        660 
RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI CIMAQMGSGI 

       670        680        690        700        710        720 
PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL DDINTETLLI LDELGRGSSI 

       730        740        750        760        770        780 
ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ 

       790        800        810        820        830        840 
LTQKSVAIEN SGIRVVKKIF NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK 

       850        860        870 
RIHNLVAILK ECAGNEKEPL TLGKLKEINS DFIENFEE 

References and documentation are available.

Number of amino acids: 878

Molecular weight: 99225.30

Theoretical pI: 6.95

Amino acid composition: 
Ala (A)  51	  5.8%
Arg (R)  37	  4.2%
Asn (N)  60	  6.8%
Asp (D)  47	  5.4%
Cys (C)  17	  1.9%
Gln (Q)  27	  3.1%
Glu (E)  62	  7.1%
Gly (G)  26	  3.0%
His (H)  15	  1.7%
Ile (I)  80	  9.1%
Leu (L) 101	 11.5%
Lys (K)  71	  8.1%
Met (M)  25	  2.8%
Phe (F)  31	  3.5%
Pro (P)  20	  2.3%
Ser (S)  88	 10.0%
Thr (T)  54	  6.2%
Trp (W)   4	  0.5%
Tyr (Y)  22	  2.5%
Val (V)  40	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 109
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      4379
Hydrogen    H	      7117
Nitrogen    N	      1181
Oxygen      O	      1348
Sulfur      S	        42

Formula: C4379H7117N1181O1348S42
Total number of atoms: 14067

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55780
Abs 0.1% (=1 g/l)   0.562, assuming all pairs of Cys residues form cystines


Ext. coefficient    54780
Abs 0.1% (=1 g/l)   0.552, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.95
This classifies the protein as unstable.



Aliphatic index: 99.42

Grand average of hydropathicity (GRAVY): -0.205