ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSESVAIIGA GLVGCLAALA FSKEGYNVTL YDFRQDPRLD TTKNKNLKSI NLAISARGID 

        70         80         90        100        110        120 
ALKSIDPDAC EHILQDMIPM KGRMIHDLKG RQESQLYGLH GEAINSINRS VLNNSLLDEL 

       130        140        150        160        170        180 
EKSTTELKFG HKLVKIEWTD DKQICHFAIG EDLKTPHTEK YDFVIGCDGA YSATRSQMQR 

       190        200        210        220        230        240 
KVEMDFSQEY MNLRYIELYI PPTEEFKPNY GGNFAIAPDH LHIWPRHKFM LIALANSDGS 

       250        260        270        280        290        300 
FTSTFFGSKD QISDLITSKS RVREFLIENF PDIINIMDLD DAVKRFITYP KESLVCVNCK 

       310        320        330        340        350        360 
PYDVPGGKAI LLGDAAHAMV PFYGQGMNCG FEDVRILMAL LKKHSGDRSR AFTEYTQTRH 

       370        380        390        400        410        420 
KDLVSITELA KRNYKEMSHD VTSKRFLLRK KLDALFSIIM KDKWIPLYTM ISFRSDISYS 

       430        440        450        460 
RALERAGKQT RILKFLESLT LGMLSIGGYK LFKFLTRERS 

References and documentation are available.

Number of amino acids: 460

Molecular weight: 52429.50

Theoretical pI: 8.60

Amino acid composition: 
Ala (A)  28	  6.1%
Arg (R)  26	  5.7%
Asn (N)  17	  3.7%
Asp (D)  32	  7.0%
Cys (C)   7	  1.5%
Gln (Q)  12	  2.6%
Glu (E)  26	  5.7%
Gly (G)  28	  6.1%
His (H)  13	  2.8%
Ile (I)  37	  8.0%
Leu (L)  48	 10.4%
Lys (K)  37	  8.0%
Met (M)  16	  3.5%
Phe (F)  24	  5.2%
Pro (P)  15	  3.3%
Ser (S)  35	  7.6%
Thr (T)  23	  5.0%
Trp (W)   3	  0.7%
Tyr (Y)  17	  3.7%
Val (V)  16	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2347
Hydrogen    H	      3711
Nitrogen    N	       633
Oxygen      O	       681
Sulfur      S	        23

Formula: C2347H3711N633O681S23
Total number of atoms: 7395

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42205
Abs 0.1% (=1 g/l)   0.805, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.798, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.06
This classifies the protein as unstable.



Aliphatic index: 88.24

Grand average of hydropathicity (GRAVY): -0.282