ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSESVKENVT PTRNFRRTQG PQNNTKPHND RKNFRRKQKK NNLSAEPNLT TSSADDTDEE 

        70         80         90        100        110        120 
NELCVICARK LTYVSLTPCH HKTCHICGFR QRALYNKKSC LICRTENEEV MFTDRIDGDI 

       130        140        150        160        170        180 
SDKYNFCEKN EKYGINFTSE EVATETLNLL KFFCPLSKDE QVCDFGSFKK YNEHLKSEHN 

       190        200        210        220        230        240 
RMICLICATH KHAFPCELEI FTQNQLRNHQ TKGNSEGFKG HPMCAFCSGK RFYSDDELYI 

       250        260        270        280        290        300 
HMRNQHEKCH ICDKMNPASP QYFKDYNQLF DHFKHSHYVC TVQTCLDNKF VVFKDELELQ 

       310        320        330        340        350        360 
AHILQEHGNI LKGKPKFFQS ELSTFISAPS RVIRERDDYD LPSISSLPGS SSGSRTDVRS 

       370        380        390        400        410        420 
ASSPEESRLR LAERAKYYLE NSKEDFNKFS SYNEDYSKGR LSAEKLLESY KLLFTKPNAD 

       430        440        450        460        470        480 
VYLLIHNLAE TFPKNSSKYN NLNAIYEQRE QTLARQTSLP SLSSDSSLSM SIGRGHWGGT 

       490        500        510        520        530        540 
NDGGSAGAAL GVRNIKNLPT LKSPSASYDP FATTVKKNTL RPVQNIKRTT PQSVSYRTST 

       550        560        570        580        590        600 
NTVAFSPTYL ESKKGSSSTS LNNSKDKLKS LNLPQLPPPK PKVQIPGLNR PQIADPKQWG 

       610        620        630 
KKSSTQDTNV HDNLRELNTT SGGNKKKGKQ KQLLFHIGV 

References and documentation are available.

Number of amino acids: 639

Molecular weight: 72755.85

Theoretical pI: 9.14

Amino acid composition: 
Ala (A)  26	  4.1%
Arg (R)  33	  5.2%
Asn (N)  49	  7.7%
Asp (D)  31	  4.9%
Cys (C)  19	  3.0%
Gln (Q)  27	  4.2%
Glu (E)  40	  6.3%
Gly (G)  29	  4.5%
His (H)  22	  3.4%
Ile (I)  24	  3.8%
Leu (L)  56	  8.8%
Lys (K)  60	  9.4%
Met (M)   7	  1.1%
Phe (F)  30	  4.7%
Pro (P)  32	  5.0%
Ser (S)  66	 10.3%
Thr (T)  42	  6.6%
Trp (W)   2	  0.3%
Tyr (Y)  22	  3.4%
Val (V)  22	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      3179
Hydrogen    H	      5006
Nitrogen    N	       920
Oxygen      O	       988
Sulfur      S	        26

Formula: C3179H5006N920O988S26
Total number of atoms: 10119

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44905
Abs 0.1% (=1 g/l)   0.617, assuming all pairs of Cys residues form cystines


Ext. coefficient    43780
Abs 0.1% (=1 g/l)   0.602, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.94
This classifies the protein as unstable.



Aliphatic index: 62.88

Grand average of hydropathicity (GRAVY): -0.842