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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSETNGGNAA RENSEVKQTA VENPIDKLDG TPKRPREKDQ DEQAEETSDK SEAPNKNDEE

70 80 90 100 110 120
KKEEGKKDQE PSHKKIKVDD GKTVESGIVE DDKKEDKFVF GAASKFGTGF GVAKKDTKDG

130 140 150 160 170 180
DATTSTESLP ASDSKTKKPF AFGSGLSFGS GFNILKNKTE NNSESEKKAT DVDKDKVHSG

190 200 210 220 230 240
SEQLANASED TKDKPKPLKL QKQEVKSGEE SEECIYQVNA KLYQLSNIKE GWKERGVGII

250 260 270 280 290 300
KINKSKDDVE KTRIVMRSRG ILKVILNIQL VKGFTVQKGF TGSLQSEKFI RLLAVDDNGD

310 320
PAQYAIKTGK KETTDELYNI IVKSVPK


References and documentation are available.

Number of amino acids: 327 Molecular weight: 36054.19 Theoretical pI: 6.33
Amino acid composition: 
Ala (A) 18 5.5% Arg (R) 8 2.4% Asn (N) 17 5.2% Asp (D) 27 8.3% Cys (C) 1 0.3% Gln (Q) 13 4.0% Glu (E) 34 10.4% Gly (G) 26 8.0% His (H) 2 0.6% Ile (I) 17 5.2% Leu (L) 16 4.9% Lys (K) 52 15.9% Met (M) 2 0.6% Phe (F) 11 3.4% Pro (P) 11 3.4% Ser (S) 27 8.3% Thr (T) 20 6.1% Trp (W) 1 0.3% Tyr (Y) 4 1.2% Val (V) 20 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 61 Total number of positively charged residues (Arg + Lys): 60 Atomic composition: Carbon C 1562 Hydrogen H 2546 Nitrogen N 438 Oxygen O 531 Sulfur S 3 Formula: C1562H2546N438O531S3 Total number of atoms: 5080 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 11460 Abs 0.1% (=1 g/l) 0.318, assuming all pairs of Cys residues form cystines Ext. coefficient 11460 Abs 0.1% (=1 g/l) 0.318, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 39.19 This classifies the protein as stable. Aliphatic index: 62.60 Grand average of hydropathicity (GRAVY): -1.048