ProtParam
User-provided sequence:
10 20 30 40 50 60
MSETNGGNAA RENSEVKQTA VENPIDKLDG TPKRPREKDQ DEQAEETSDK SEAPNKNDEE
70 80 90 100 110 120
KKEEGKKDQE PSHKKIKVDD GKTVESGIVE DDKKEDKFVF GAASKFGTGF GVAKKDTKDG
130 140 150 160 170 180
DATTSTESLP ASDSKTKKPF AFGSGLSFGS GFNILKNKTE NNSESEKKAT DVDKDKVHSG
190 200 210 220 230 240
SEQLANASED TKDKPKPLKL QKQEVKSGEE SEECIYQVNA KLYQLSNIKE GWKERGVGII
250 260 270 280 290 300
KINKSKDDVE KTRIVMRSRG ILKVILNIQL VKGFTVQKGF TGSLQSEKFI RLLAVDDNGD
310 320
PAQYAIKTGK KETTDELYNI IVKSVPK
References and
documentation are available.
Number of amino acids: 327
Molecular weight: 36054.19
Theoretical pI: 6.33
Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 1562
Hydrogen H 2546
Nitrogen N 438
Oxygen O 531
Sulfur S 3
Formula: C
1562H
2546N
438O
531S
3
Total number of atoms: 5080
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.318, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.318, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.19
This classifies the protein as stable.
Aliphatic index: 62.60
Grand average of hydropathicity (GRAVY): -1.048