ProtParam
User-provided sequence:
10 20 30 40 50 60
MSETRESNES TVSSLQTKLL LNDGISENNK KNVILLNQIV PFILNTSHYM TDLMYVLYYL
70 80 90 100 110 120
AQKQEDEVLN HSGTFISHKK ELLALKSDIC ELIYDLRTGF RLLLDSCELD HFETPGKCRH
130 140 150 160 170 180
LIEKVLVTSI YGVNRYIFQE LNRLNVDFKD EFILQMQNCL SGFVNLYKFL NKIPMSKQQS
190 200 210 220 230 240
QMNDLQMKIL VNVLQNELLP IWKFQLDLLN CKLFNELSKD KGLINIYRKA TNDSVIDVSK
250 260
GEPFIRYVNW LKDQIIGEMT V
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 30473.36
Theoretical pI: 6.00
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1372
Hydrogen H 2186
Nitrogen N 354
Oxygen O 401
Sulfur S 13
Formula: C
1372H
2186N
354O
401S
13
Total number of atoms: 4326
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26150
Abs 0.1% (=1 g/l) 0.858, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.850, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.58
This classifies the protein as unstable.
Aliphatic index: 108.58
Grand average of hydropathicity (GRAVY): -0.170