ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSETRESNES TVSSLQTKLL LNDGISENNK KNVILLNQIV PFILNTSHYM TDLMYVLYYL 

        70         80         90        100        110        120 
AQKQEDEVLN HSGTFISHKK ELLALKSDIC ELIYDLRTGF RLLLDSCELD HFETPGKCRH 

       130        140        150        160        170        180 
LIEKVLVTSI YGVNRYIFQE LNRLNVDFKD EFILQMQNCL SGFVNLYKFL NKIPMSKQQS 

       190        200        210        220        230        240 
QMNDLQMKIL VNVLQNELLP IWKFQLDLLN CKLFNELSKD KGLINIYRKA TNDSVIDVSK 

       250        260 
GEPFIRYVNW LKDQIIGEMT V 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 30473.36

Theoretical pI: 6.00

Amino acid composition: 
Ala (A)   3	  1.1%
Arg (R)   8	  3.1%
Asn (N)  22	  8.4%
Asp (D)  15	  5.7%
Cys (C)   5	  1.9%
Gln (Q)  14	  5.4%
Glu (E)  17	  6.5%
Gly (G)   9	  3.4%
His (H)   5	  1.9%
Ile (I)  20	  7.7%
Leu (L)  40	 15.3%
Lys (K)  21	  8.0%
Met (M)   8	  3.1%
Phe (F)  12	  4.6%
Pro (P)   5	  1.9%
Ser (S)  18	  6.9%
Thr (T)  11	  4.2%
Trp (W)   2	  0.8%
Tyr (Y)  10	  3.8%
Val (V)  16	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1372
Hydrogen    H	      2186
Nitrogen    N	       354
Oxygen      O	       401
Sulfur      S	        13

Formula: C1372H2186N354O401S13
Total number of atoms: 4326

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26150
Abs 0.1% (=1 g/l)   0.858, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.850, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.58
This classifies the protein as unstable.



Aliphatic index: 108.58

Grand average of hydropathicity (GRAVY): -0.170