ProtParam
User-provided sequence:
10 20 30 40 50 60
MSETRMAQNM DTTDEQYLRL IELLSNYDST LEQLQKGFQD GYIQLSRSNY YNKDSLRGNY
70 80 90 100 110 120
GEDYWDETYI GQLMATVEEK NSKVVVEIVK RKAQDKQEKK EEEDNKLTQR KKGTKPEKQK
130 140 150 160 170 180
TQSHKLKQDY DPILMFGGVL SVPSSLRQSQ TSFKGCIPLI AQLINYKNEI LTLVETLSEQ
E
References and
documentation are available.
Number of amino acids: 181
Molecular weight: 21077.72
Theoretical pI: 5.41
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 921
Hydrogen H 1478
Nitrogen N 250
Oxygen O 302
Sulfur S 6
Formula: C
921H
1478N
250O
302S
6
Total number of atoms: 2957
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.968, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.968, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 62.77
This classifies the protein as unstable.
Aliphatic index: 75.36
Grand average of hydropathicity (GRAVY): -0.944