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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSETRMAQNM DTTDEQYLRL IELLSNYDST LEQLQKGFQD GYIQLSRSNY YNKDSLRGNY

70 80 90 100 110 120
GEDYWDETYI GQLMATVEEK NSKVVVEIVK RKAQDKQEKK EEEDNKLTQR KKGTKPEKQK

130 140 150 160 170 180
TQSHKLKQDY DPILMFGGVL SVPSSLRQSQ TSFKGCIPLI AQLINYKNEI LTLVETLSEQ


E


References and documentation are available.

Number of amino acids: 181 Molecular weight: 21077.72 Theoretical pI: 5.41
Amino acid composition: 
Ala (A) 4 2.2% Arg (R) 7 3.9% Asn (N) 9 5.0% Asp (D) 11 6.1% Cys (C) 1 0.6% Gln (Q) 17 9.4% Glu (E) 18 9.9% Gly (G) 9 5.0% His (H) 1 0.6% Ile (I) 9 5.0% Leu (L) 19 10.5% Lys (K) 19 10.5% Met (M) 5 2.8% Phe (F) 3 1.7% Pro (P) 4 2.2% Ser (S) 14 7.7% Thr (T) 12 6.6% Trp (W) 1 0.6% Tyr (Y) 10 5.5% Val (V) 8 4.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 29 Total number of positively charged residues (Arg + Lys): 26 Atomic composition: Carbon C 921 Hydrogen H 1478 Nitrogen N 250 Oxygen O 302 Sulfur S 6 Formula: C921H1478N250O302S6 Total number of atoms: 2957 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 20400 Abs 0.1% (=1 g/l) 0.968, assuming all pairs of Cys residues form cystines Ext. coefficient 20400 Abs 0.1% (=1 g/l) 0.968, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 62.77 This classifies the protein as unstable. Aliphatic index: 75.36 Grand average of hydropathicity (GRAVY): -0.944