ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEVAPEEII ENADGSRSII TYKIEDGVKY KITQKVKEVK VLEKVHKSVA ERKNWHKYGS 

        70         80         90        100        110        120 
EKGSPAGPSA VTARLGEEVE LRLSRNWKQA EEERIQKEKA SLTKTGLQCR LCGNDHMTMN 

       130        140        150        160        170        180 
CPFKTILSEL SALEDPATNE GGVEAASEEK AGQVGGAGSI PGQYVPPSRR AGARDPSSDA 

       190        200        210        220        230        240 
YRDSRERDDM CTLKIMQVNE NADENSLREE LLFPFAPIPR VSVVRNKETG KSRGLAFVTF 

       250        260        270 
SSEEVAEQAL RFLDGRGYMN LILRVEWSKP KVKE 

References and documentation are available.

Number of amino acids: 274

Molecular weight: 30501.47

Theoretical pI: 6.41

Amino acid composition: 
Ala (A)  22	  8.0%
Arg (R)  20	  7.3%
Asn (N)  11	  4.0%
Asp (D)  11	  4.0%
Cys (C)   4	  1.5%
Gln (Q)   8	  2.9%
Glu (E)  33	 12.0%
Gly (G)  20	  7.3%
His (H)   3	  1.1%
Ile (I)  12	  4.4%
Leu (L)  19	  6.9%
Lys (K)  23	  8.4%
Met (M)   6	  2.2%
Phe (F)   6	  2.2%
Pro (P)  13	  4.7%
Ser (S)  23	  8.4%
Thr (T)  11	  4.0%
Trp (W)   3	  1.1%
Tyr (Y)   6	  2.2%
Val (V)  20	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1322
Hydrogen    H	      2141
Nitrogen    N	       385
Oxygen      O	       422
Sulfur      S	        10

Formula: C1322H2141N385O422S10
Total number of atoms: 4280

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25690
Abs 0.1% (=1 g/l)   0.842, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   0.834, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.12
This classifies the protein as unstable.



Aliphatic index: 73.32

Grand average of hydropathicity (GRAVY): -0.683