ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEVSKWPAI NPFHWGYNGT VSHIVGENGS IKLHLKDNKE QVDFDEFANK YVPTLKNGAQ
70 80 90 100 110 120
FKLSPYLFTG ILQTLYLGAA DFSKKFPVFY GREIVKFSDG GVCTADWLID SWKKDYEFDQ
130 140 150 160 170 180
STTSFDKKKF DKDEKATHPE GWPRLQPRTR YLKDNELEEL REVDLPLVVI LHGLAGGSHE
190 200 210 220 230 240
PIIRSLAENL SRSGRFQVVV LNTRGCARSK ITTRNLFTAY HTMDIREFLQ REKQRHPDRK
250 260 270 280 290 300
LYAVGCSFGA TMLANYLGEE GDKSPLSAAA TLCNPWDLLL SAIRMSQDWW SRTLFSKNIA
310 320 330 340 350 360
QFLTRTVQVN MGELGVPNGS LPDHPPTVKN PSFYMFTPEN LIKAKSFKST REFDEVYTAP
370 380 390 400 410 420
ALGFPNAMEY YKAASSINRV DTIRVPTLVI NSRDDPVVGP DQPYSIVEKN PRILYCRTDL
430 440 450
GGHLAYLDKD NNSWATKAIA EFFTKFDELV V
References and
documentation are available.
Number of amino acids: 451
Molecular weight: 51255.25
Theoretical pI: 7.64
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2318
Hydrogen H 3563
Nitrogen N 619
Oxygen O 673
Sulfur S 12
Formula: C
2318H
3563N
619O
673S
12
Total number of atoms: 7185
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 75080
Abs 0.1% (=1 g/l) 1.465, assuming all pairs of Cys residues form cystines
Ext. coefficient 74830
Abs 0.1% (=1 g/l) 1.460, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.91
This classifies the protein as stable.
Aliphatic index: 78.05
Grand average of hydropathicity (GRAVY): -0.419