ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD 

        70         80         90        100        110        120 
TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW 

       130        140        150        160        170        180 
TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK 

       190        200        210        220        230        240 
KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR 

       250        260        270        280 
RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R 

References and documentation are available.

Number of amino acids: 281

Molecular weight: 31310.15

Theoretical pI: 5.08

Amino acid composition: 
Ala (A)  19	  6.8%
Arg (R)  13	  4.6%
Asn (N)  13	  4.6%
Asp (D)  22	  7.8%
Cys (C)   0	  0.0%
Gln (Q)   8	  2.8%
Glu (E)  24	  8.5%
Gly (G)   6	  2.1%
His (H)   4	  1.4%
Ile (I)   9	  3.2%
Leu (L)  27	  9.6%
Lys (K)  23	  8.2%
Met (M)   5	  1.8%
Phe (F)  11	  3.9%
Pro (P)  20	  7.1%
Ser (S)  31	 11.0%
Thr (T)  20	  7.1%
Trp (W)   1	  0.4%
Tyr (Y)   4	  1.4%
Val (V)  21	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1374
Hydrogen    H	      2205
Nitrogen    N	       373
Oxygen      O	       450
Sulfur      S	         5

Formula: C1374H2205N373O450S5
Total number of atoms: 4407

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.366

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.02
This classifies the protein as unstable.



Aliphatic index: 78.40

Grand average of hydropathicity (GRAVY): -0.601