ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFDINWSTL ESDNRLNDLI RKHLNSYLQN TQLPSYVSNL RVLDFDLGKV GPAITLKEIT
70 80 90 100 110 120
DPLDEFYDSI REEADQETEE NNDNKEDSEH ICPDRTIANH EGPKDDFEAP VVMPSPNDIQ
130 140 150 160 170 180
FLLEVEYKGD LLVTIGADLV LNYPVEKFMT LPVKLSISDI GLHSLCIVAC LSKQLFLSFL
190 200 210 220 230 240
CDVSDPALDD NQTVLDPKGP ILAATKPLER ISIVRSMKIE TEIGEQYQGQ GSVLRSVGEL
250 260 270
EQFLFTIFKD FLRKELAWPS WINLDFNDGD E
References and
documentation are available.
Number of amino acids: 271
Molecular weight: 30728.65
Theoretical pI: 4.31
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1372
Hydrogen H 2142
Nitrogen N 348
Oxygen O 435
Sulfur S 8
Formula: C
1372H
2142N
348O
435S
8
Total number of atoms: 4305
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25690
Abs 0.1% (=1 g/l) 0.836, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.828, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.72
This classifies the protein as unstable.
Aliphatic index: 98.52
Grand average of hydropathicity (GRAVY): -0.282