ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFFDIEAQS SKGNSQQEPQ FSTNQKTKEL SNLIETFAEQ SRVLEKECTK IGSKRDSKEL 

        70         80         90        100        110        120 
RYKIETELIP NCTSVRDKIE SNILIHQNGK LSADFKNLKT KYQSLQQSYN QRKSLFPLKT 

       130        140        150        160        170        180 
PISPGTSKER KDIHPRTEAV RQDPESSYIS IKVNEQSPLL HNEGQHQLQL QEEQEQQQQG 

       190        200        210        220        230        240 
LSQEELDFQT IIHQERSQQI GRIHTAVQEV NAIFHQLGSL VKEQGEQVTT IDENISHLHD 

       250        260        270        280 
NMQNANKQLT RADQHQRDRN KCGKVTLIII IVVCMVVLLA VLS 

References and documentation are available.

Number of amino acids: 283

Molecular weight: 32497.54

Theoretical pI: 6.57

Amino acid composition: 
Ala (A)   9	  3.2%
Arg (R)  13	  4.6%
Asn (N)  16	  5.7%
Asp (D)  11	  3.9%
Cys (C)   4	  1.4%
Gln (Q)  35	 12.4%
Glu (E)  26	  9.2%
Gly (G)  10	  3.5%
His (H)  10	  3.5%
Ile (I)  23	  8.1%
Leu (L)  25	  8.8%
Lys (K)  22	  7.8%
Met (M)   3	  1.1%
Phe (F)   8	  2.8%
Pro (P)   8	  2.8%
Ser (S)  26	  9.2%
Thr (T)  16	  5.7%
Trp (W)   0	  0.0%
Tyr (Y)   4	  1.4%
Val (V)  14	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1405
Hydrogen    H	      2287
Nitrogen    N	       415
Oxygen      O	       455
Sulfur      S	         7

Formula: C1405H2287N415O455S7
Total number of atoms: 4569

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6210
Abs 0.1% (=1 g/l)   0.191, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.183, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 65.49
This classifies the protein as unstable.



Aliphatic index: 83.67

Grand average of hydropathicity (GRAVY): -0.801