ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFFKKKLSK GKEILSKSNL KTHTSSNASL SIDDLNRFGF SLNPVLWCLD HQQGLLAIVS 

        70         80         90        100        110        120 
STNRIYIYGK QHVQSVIVPD CSTIVHIALC AAYLIVIDSR NTVLSYPLMK HRDLSKPAAT 

       130        140        150        160        170        180 
YFLKQKVTCT VTDPTIDWVF FGMSDGSVVP WDVTRHCLGK FKVPNLYVPR HEEWRMMGYS 

       190        200        210        220        230        240 
YAPVPGKLSP VVSVQIHPKD LGVILIAYPD GVVLYSIRTD EVIRFYELEY APGSTAAVLS 

       250        260        270        280        290        300 
PHNYRRPIVK GIEWSPWGDH FVSYYTDSTF AFWDVDQEYP VQVRNFVDSN IHTYTPMQRN 

       310        320        330        340        350        360 
PPKTELEPIR SMRWCCCEDP TVSFILMLGG LPKEAPVKGI SLFSYRNLPA KKDVETFAEF 

       370        380        390        400        410        420 
FANPNSQRFF PFIDIPPVRD MLVIPSSSPH YNGSHNPKNL LLLSEDNSLS LLDISTGNIS 

       430        440        450        460        470        480 
NMSLSIPPSL CFLASDFRVI AFQTVTKKVW NQIEDTISVN SHYSCLFGGS PSPGYLKKLD 

       490        500        510        520        530        540 
ERNLLITSTG LSLSIWDISQ GFMNPSLCVN LDFSSVMRKH LTPSAFITTA SFSTYNPEFS 

       550        560        570        580        590        600 
CADSFGRVIV CKRKNHKENL PAQLANGIYR LDDTLVLEGT LHAQYYIDLK RGRVTLNQMS 

       610        620        630        640        650        660 
NIGFVCIGYQ DGGITIIDMR GPHILCNTSI SELGLERRGK PDPDFLTSAE FVVMNPKGSP 

       670        680        690        700        710        720 
SSIYVVTGTY RGMTLLFRID PSSSGRFSAY FESSRQLDIK NIYKICSLTQ DGQIATATGS 

       730        740        750        760        770        780 
SLQSVGYPLP QEVFLVYIGD SGISVFNKIN NQVGNLDWRK PVCCRAALVL STVSKHMGSV 

       790        800        810        820        830        840 
VCVNSDLSVN WYSLPNLREE RKMQLPLDID KNRLKEGDIL GNGDYIFPTL GAHELAFGCV 

       850        860        870        880        890        900 
LGSGRTLANL APMMLITHNA SHVPPRPSKS LWNWLLGEQS TSAEELDILL GGENRAESKV 

       910        920        930        940        950        960 
HTLETPKVIS ARPAESVKQP LTPVPSMTSQ SAQSYIPPRR QQQQKGFFAQ INDHLAQRGN 

       970        980        990       1000 
MLGGIENTMD DLEEMSAEWA NEIKDSLAGT KKDLILSGLK SYIP 

References and documentation are available.

Number of amino acids: 1004

Molecular weight: 112010.39

Theoretical pI: 7.76

Amino acid composition: 
Ala (A)  46	  4.6%
Arg (R)  45	  4.5%
Asn (N)  51	  5.1%
Asp (D)  53	  5.3%
Cys (C)  20	  2.0%
Gln (Q)  34	  3.4%
Glu (E)  41	  4.1%
Gly (G)  62	  6.2%
His (H)  24	  2.4%
Ile (I)  66	  6.6%
Leu (L) 101	 10.1%
Lys (K)  51	  5.1%
Met (M)  24	  2.4%
Phe (F)  43	  4.3%
Pro (P)  65	  6.5%
Ser (S) 105	 10.5%
Thr (T)  53	  5.3%
Trp (W)  15	  1.5%
Tyr (Y)  36	  3.6%
Val (V)  69	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 96

Atomic composition:

Carbon      C	      5028
Hydrogen    H	      7854
Nitrogen    N	      1338
Oxygen      O	      1472
Sulfur      S	        44

Formula: C5028H7854N1338O1472S44
Total number of atoms: 15736

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   137390
Abs 0.1% (=1 g/l)   1.227, assuming all pairs of Cys residues form cystines


Ext. coefficient   136140
Abs 0.1% (=1 g/l)   1.215, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.99
This classifies the protein as unstable.



Aliphatic index: 89.38

Grand average of hydropathicity (GRAVY): -0.145