ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF GEPDLTKGEQ
70 80 90 100
CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA KEIADDSKK
References and
documentation are available.
Number of amino acids: 109
Molecular weight: 12283.95
Theoretical pI: 6.27
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 533
Hydrogen H 834
Nitrogen N 152
Oxygen O 164
Sulfur S 9
Formula: C
533H
834N
152O
164S
9
Total number of atoms: 1692
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 3355
Abs 0.1% (=1 g/l) 0.273, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.243, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.08
This classifies the protein as unstable.
Aliphatic index: 67.06
Grand average of hydropathicity (GRAVY): -0.412