ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFFNFKAFG RNSKKNKNQP LNVAQPPAMN TIYSSPHSSN SRLSLRNKHH SPKRHSQTSF
70 80 90 100 110 120
PAQKSTPQSQ QLTSTTPQSQ QQEASERSES QQIMFLSEPF VRTALVKGSF KTIVQLPKYV
130 140 150 160 170 180
DLGEWIALNV FEFFTNLNQF YGVVAEYVTP DAYPTMNAGP HTDYLWLDAN NRQVSLPASQ
190 200 210 220 230 240
YIDLALTWIN NKVNDKNLFP TKNGLPFPQQ FSRDVQRIMV QMFRIFAHIY HHHFDKIVHL
250 260 270 280
SLEAHWNSFF SHFISFAKEF KIIDRKEMAP LLPLIESFEK QGKIIYN
References and
documentation are available.
Number of amino acids: 287
Molecular weight: 33275.84
Theoretical pI: 9.60
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1516
Hydrogen H 2298
Nitrogen N 410
Oxygen O 424
Sulfur S 7
Formula: C
1516H
2298N
410O
424S
7
Total number of atoms: 4655
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35410
Abs 0.1% (=1 g/l) 1.064
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.21
This classifies the protein as unstable.
Aliphatic index: 71.71
Grand average of hydropathicity (GRAVY): -0.492