ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFGSKVSRA LRFLEIPVKD RASVSFLKNP DLQPIKSANQ TWGFWSNFAY WGVMSFSVGT 

        70         80         90        100        110        120 
WMSASSALGV GLSYPETIGT FIVGDVLTII FTLANSCPGY DWKVGFTLAQ RFVFGIYGSA 

       130        140        150        160        170        180 
FGIIIRILMS IVNYGSNAWV GGLCINMILD SWSHHYLHLP NTLSSKVAMT TKELIGFIIF 

       190        200        210        220        230        240 
HVLTAFCYLM KPYHMNYILI WSCVATFFSM LGMVIYLAKQ AHGVGELFTS TKSTATGSTK 

       250        260        270        280        290        300 
AWAWVYMISY WFGSVSPGST NQSDYSRFGS SNWAIWAGTI CALLIPTTLI PVFGVIGAST 

       310        320        330        340        350        360 
CDKLYGEQYW MPMDIFNHWL TTNYSAGARA GAFFCGLSFV LSQMSYTISN CGFASGMDLA 

       370        380        390        400        410        420 
GLLPKYVDIK RGALFAACVS WACLPWNFYN SSSTFLTVMS SFGVVMTPII SVMICDNFLI 

       430        440        450        460        470        480 
RKRQYSITNA FILKGEYYFT KGVNWRAIVA WVCGMTPGLP GIAWEVNNDY FHNTGIVNFF 

       490        500        510        520        530        540 
YGDSFFSFLI SFFVYWGLCL LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI 

       550        560        570        580        590 
RAYTLGEGYT TGHEYRPEGS DDEIPELVKT SSENTNEFEI VHHKNNEKQS STASEKAA 

References and documentation are available.

Number of amino acids: 598

Molecular weight: 66903.92

Theoretical pI: 6.63

Amino acid composition: 
Ala (A)  42	  7.0%
Arg (R)  14	  2.3%
Asn (N)  27	  4.5%
Asp (D)  19	  3.2%
Cys (C)  13	  2.2%
Gln (Q)   9	  1.5%
Glu (E)  23	  3.8%
Gly (G)  50	  8.4%
His (H)  12	  2.0%
Ile (I)  44	  7.4%
Leu (L)  44	  7.4%
Lys (K)  26	  4.3%
Met (M)  20	  3.3%
Phe (F)  45	  7.5%
Pro (P)  20	  3.3%
Ser (S)  60	 10.0%
Thr (T)  40	  6.7%
Trp (W)  21	  3.5%
Tyr (Y)  31	  5.2%
Val (V)  38	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      3094
Hydrogen    H	      4585
Nitrogen    N	       747
Oxygen      O	       850
Sulfur      S	        33

Formula: C3094H4585N747O850S33
Total number of atoms: 9309

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   162440
Abs 0.1% (=1 g/l)   2.428, assuming all pairs of Cys residues form cystines


Ext. coefficient   161690
Abs 0.1% (=1 g/l)   2.417, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.96
This classifies the protein as stable.



Aliphatic index: 82.84

Grand average of hydropathicity (GRAVY): 0.224