ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFGTRISRA LRFLEIPVKN RASVNFLRNP DLQPIKSVNQ TWGFWSNFAY WGVLSFNVGM 

        70         80         90        100        110        120 
WIGGSSALTV GLSYSETIGA FIIADLLTIL FALANSCPGY DWKVGFTLAQ RFVFGIYGSA 

       130        140        150        160        170        180 
LGIIIRILMS IVYYGSNAWL GGLCVNMILD SWSHHYLHLP NTLSSKVAMT TKELIGFIIF 

       190        200        210        220        230        240 
HILTAFCYFM KPYHMNYILI WSCVGTFFAM LGMVIYLTKS AHGVGDLFTS THSTVTGSKK 

       250        260        270        280        290        300 
AWAWVYTISY WYGSVSPGCT NQSDFSRFGS SNCAIWTGTI VALLIPATLI PVFGIIGASA 

       310        320        330        340        350        360 
CEKLYGQTFW MPMDIFDNWL TTNYSAGARA ATFFCGFCFV MSQISYTISN CGFASGMDLA 

       370        380        390        400        410        420 
GLLPKYVDIK RGAIFAACVS WACLPWNFYN SSSTFLTVMS SFGVVMTPII TVMICDNFLI 

       430        440        450        460        470        480 
RKRQYSVTNA FVLKGEYYFT KGVNWRAIVA WVCGMAPGLP GIAWEVNNDY FHNTGIINFF 

       490        500        510        520        530        540 
YGDSFFSFLI SFFVYWGLCL LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI 

       550        560        570        580        590 
RAYTLGECFT SGHEYKPESS DDELPELTKT SSENTKVFEI VHQKDNEKES STSSEKQIA 

References and documentation are available.

Number of amino acids: 599

Molecular weight: 67185.50

Theoretical pI: 6.81

Amino acid composition: 
Ala (A)  41	  6.8%
Arg (R)  15	  2.5%
Asn (N)  27	  4.5%
Asp (D)  20	  3.3%
Cys (C)  16	  2.7%
Gln (Q)   9	  1.5%
Glu (E)  22	  3.7%
Gly (G)  48	  8.0%
His (H)  11	  1.8%
Ile (I)  48	  8.0%
Leu (L)  46	  7.7%
Lys (K)  26	  4.3%
Met (M)  18	  3.0%
Phe (F)  48	  8.0%
Pro (P)  19	  3.2%
Ser (S)  57	  9.5%
Thr (T)  41	  6.8%
Trp (W)  20	  3.3%
Tyr (Y)  30	  5.0%
Val (V)  37	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      3113
Hydrogen    H	      4624
Nitrogen    N	       748
Oxygen      O	       848
Sulfur      S	        34

Formula: C3113H4624N748O848S34
Total number of atoms: 9367

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   155700
Abs 0.1% (=1 g/l)   2.317, assuming all pairs of Cys residues form cystines


Ext. coefficient   154700
Abs 0.1% (=1 g/l)   2.303, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.67
This classifies the protein as stable.



Aliphatic index: 85.96

Grand average of hydropathicity (GRAVY): 0.285