ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFHQQFFTL NNGNKIPAIA IIGTGTRWYK NEETDATFSN SLVEQIVYAL KLPGIIHIDA
70 80 90 100 110 120
AEIYRTYPEV GKALSLTEKP RNAIFLTDKY SPQIKMSDSP ADGLDLALKK MGTDYVDLYL
130 140 150 160 170 180
LHSPFVSKEV NGLSLEEAWK DMEQLYKSGK AKNIGVSNFA VEDLQRILKV AEVKPQVNQI
190 200 210 220 230 240
EFSPFLQNQT PGIYKFCQEH DILVEAYSPL GPLQKKTAQD DSQPFFEYVK ELSEKYIKSE
250 260 270 280 290 300
AQIILRWVTK RGVLPVTTSS KPQRISDAQN LFSFDLTAEE VDKITELGLE HEPLRLYWNK
310
LYGKYNYAAQ KV
References and
documentation are available.
Number of amino acids: 312
Molecular weight: 35560.60
Theoretical pI: 5.85
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1621
Hydrogen H 2520
Nitrogen N 410
Oxygen O 478
Sulfur S 5
Formula: C
1621H
2520N
410O
478S
5
Total number of atoms: 5034
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.289, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.289, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.57
This classifies the protein as unstable.
Aliphatic index: 89.71
Grand average of hydropathicity (GRAVY): -0.379