ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFIQRRLLS QTLFLRSQVG SLPLYISPEV QVSINALSMP RIIRKGRTSM NISQNITVKG
70 80 90 100 110 120
PKGELSVEVP DFLHLDKDEK HGKINVTVQN SEDKHQRSMW GTVRSLINNH IIGVTEGHLA
130 140 150 160 170 180
VLRFVGTGYR AQLENDGKFV NVKVGASIKQ GLDVPEGIVV KTPAPTSLII EGCNKQQVLL
190 200 210
FAAKLRKFHP PEPYKGKGIY VNDETIKLKD KKIK
References and
documentation are available.
Number of amino acids: 214
Molecular weight: 23888.85
Theoretical pI: 9.90
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1070
Hydrogen H 1755
Nitrogen N 303
Oxygen O 304
Sulfur S 5
Formula: C
1070H
1755N
303O
304S
5
Total number of atoms: 3437
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.480, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.480, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.36
This classifies the protein as stable.
Aliphatic index: 99.63
Grand average of hydropathicity (GRAVY): -0.282