ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFKGFTKAV SRAPQSFRQK FKMGEQTEDP VYEDAERRFQ ELEQETKKLS EESKRYSTAV
70 80 90 100 110 120
NGMLTHQIGF AKSMEEIFKP ISGKMSDPNA TIPEDNPQGI EASEQYRAIV AELQETLKPD
130 140 150 160 170 180
LALVEEKIVT PCQELLKIIT YIRKMATKRN HKKLDLDRHL NTYNKHEKKK EPTAKDEERL
190 200 210 220 230 240
YKAQAQVEVA QQEYDYYNDL LKTQLPILFS LEAEFVKPLF VSFYFMQLNI FYTLYNRLQD
250 260 270 280 290 300
MKIPYFDLNS DIVESYIAKK GNVEEQTDAL TITHFKLGYS KAKLEMTRRK YGVATAEGSP
310 320 330 340 350 360
VSGASSGVGY GAGYDPATAT SPTPTGYGYG AAAPSYAAQP AAQYGTAAAV GTAAAVGTAA
370 380 390 400 410 420
GAAAGAVPGT YPQYAAAQSP PLTGLGFQQS PQQQQGPPPA YSNPLTSPVA GTPAAAVAAA
430 440 450 460 470 480
PGVETVTALY DYQAQAAGDL SFPAGAVIEI VQRTPDVNEW WTGRYNGQQG VFPGNYVQLN
KN
References and
documentation are available.
Number of amino acids: 482
Molecular weight: 52774.34
Theoretical pI: 5.77
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2363
Hydrogen H 3653
Nitrogen N 623
Oxygen O 729
Sulfur S 10
Formula: C
2363H
3653N
623O
729S
10
Total number of atoms: 7378
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54210
Abs 0.1% (=1 g/l) 1.027, assuming all pairs of Cys residues form cystines
Ext. coefficient 54210
Abs 0.1% (=1 g/l) 1.027, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.25
This classifies the protein as unstable.
Aliphatic index: 69.36
Grand average of hydropathicity (GRAVY): -0.480