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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFKTHIKFV VVTIIYFAHI YFRRLPITLL PLIREEIVLT SDDIGILVSS HALLYTIGRL

70 80 90 100 110 120
LFGMASDRYS KVALLLLGLV TCAACSVGLA FSNHLWQMLI AMMLLGIVQG AGWVPATLLV

130 140 150 160 170 180
QSWYSKSTYG TMFSILACGS TFAGILQPFS KKFYWRNVEL YTGAGMLVFS IVCFIVLRED

190 200 210 220 230 240
NVSPKQTEDD EEPNPEKKKK GGLRTIVGSI VIWHISFVYL FTMEMRTICE TWVQLFLTDS

250 260 270 280 290 300
KISPDAFQIT YEIGGLVGTM ASGIVIDLAT RKFDVDATRR VIAVSYTCLM MFSAAGIFEF

310 320 330 340 350 360
AELSSIFGFL AGMFVNGSIN VWCMIASQAG FSKIQGTVSA FISFIASSGS MLAGTPLAYL

370 380 390 400 410 420
ISVFGWKVFL YIFSVQLVIV LFVSSFRVRL RMDTTENEAS KNEIDDDESS GVCSTVVDSQ

430 440 450
RNLTSLNKKT RISMLLALYA TKKIKPFRDV LASNKV


References and documentation are available.

Number of amino acids: 456 Molecular weight: 50635.59 Theoretical pI: 8.78
Amino acid composition: 
Ala (A) 32 7.0% Arg (R) 18 3.9% Asn (N) 11 2.4% Asp (D) 17 3.7% Cys (C) 8 1.8% Gln (Q) 11 2.4% Glu (E) 17 3.7% Gly (G) 30 6.6% His (H) 5 1.1% Ile (I) 40 8.8% Leu (L) 49 10.7% Lys (K) 22 4.8% Met (M) 17 3.7% Phe (F) 32 7.0% Pro (P) 10 2.2% Ser (S) 45 9.9% Thr (T) 30 6.6% Trp (W) 8 1.8% Tyr (Y) 14 3.1% Val (V) 40 8.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 34 Total number of positively charged residues (Arg + Lys): 40 Atomic composition: Carbon C 2328 Hydrogen H 3656 Nitrogen N 572 Oxygen O 636 Sulfur S 25 Formula: C2328H3656N572O636S25 Total number of atoms: 7217 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 65360 Abs 0.1% (=1 g/l) 1.291, assuming all pairs of Cys residues form cystines Ext. coefficient 64860 Abs 0.1% (=1 g/l) 1.281, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 33.27 This classifies the protein as stable. Aliphatic index: 108.57 Grand average of hydropathicity (GRAVY): 0.536