ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFKTHIKFV VVTIIYFAHI YFRRLPITLL PLIREEIVLT SDDIGILVSS HALLYTIGRL
70 80 90 100 110 120
LFGMASDRYS KVALLLLGLV TCAACSVGLA FSNHLWQMLI AMMLLGIVQG AGWVPATLLV
130 140 150 160 170 180
QSWYSKSTYG TMFSILACGS TFAGILQPFS KKFYWRNVEL YTGAGMLVFS IVCFIVLRED
190 200 210 220 230 240
NVSPKQTEDD EEPNPEKKKK GGLRTIVGSI VIWHISFVYL FTMEMRTICE TWVQLFLTDS
250 260 270 280 290 300
KISPDAFQIT YEIGGLVGTM ASGIVIDLAT RKFDVDATRR VIAVSYTCLM MFSAAGIFEF
310 320 330 340 350 360
AELSSIFGFL AGMFVNGSIN VWCMIASQAG FSKIQGTVSA FISFIASSGS MLAGTPLAYL
370 380 390 400 410 420
ISVFGWKVFL YIFSVQLVIV LFVSSFRVRL RMDTTENEAS KNEIDDDESS GVCSTVVDSQ
430 440 450
RNLTSLNKKT RISMLLALYA TKKIKPFRDV LASNKV
References and
documentation are available.
Number of amino acids: 456
Molecular weight: 50635.59
Theoretical pI: 8.78
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2328
Hydrogen H 3656
Nitrogen N 572
Oxygen O 636
Sulfur S 25
Formula: C
2328H
3656N
572O
636S
25
Total number of atoms: 7217
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65360
Abs 0.1% (=1 g/l) 1.291, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 1.281, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.27
This classifies the protein as stable.
Aliphatic index: 108.57
Grand average of hydropathicity (GRAVY): 0.536