ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFLGFGGGQ PQLSSQQKIQ AAEAELDLVT DMFNKLVNNC YKKCINTSYS EGELNKNESS
70 80 90
CLDRCVAKYF ETNVQVGENM QKMGQSFNAA GKF
References and
documentation are available.
Number of amino acids: 93
Molecular weight: 10304.61
Theoretical pI: 5.31
Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 9
Atomic composition:
Carbon C 444
Hydrogen H 695
Nitrogen N 121
Oxygen O 145
Sulfur S 8
Formula: C
444H
695N
121O
145S
8
Total number of atoms: 1413
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4720
Abs 0.1% (=1 g/l) 0.458, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.434, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.47
This classifies the protein as unstable.
Aliphatic index: 59.78
Grand average of hydropathicity (GRAVY): -0.490