ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFLLLTPPP CLLIPPPPLS HRRSSSLFLK HPFQPSPRPL SFCKPSALRL RANTTVNSLK 

        70         80         90        100        110        120 
ALETIKPYLQ SESKTVLLGW LCSCVSVVSL SQIVPRLGSF TSNLNANAAS LTKLKGECLV 

       130        140        150        160        170        180 
LAGLVLAKVV AYYLQQAFLW EAALNTVYKI RVFAYRRVLE RELEFFEGGN GISSGDIAYR 

       190        200        210        220        230        240 
ITAEASEVAD TIYALLNTVV PSAIQISVMT AHMIVASPAL TLVSAMVIPS VALLIAYLGD 

       250        260        270        280        290        300 
RLRKISRKAQ IASAQLSTYL NEVLPAILFV KANNAEISES VRFQRFARAD LDERFKKKKM 

       310        320        330        340        350        360 
KSLIPQIVQV MYLGSLSIFC VGAVILAGSS LSSSAIVSFV ASLAFLIDPV QDLGKAYNEL 

       370        380        390        400        410        420 
KQGEPAIERL FDLTSLESKV IERPEAIQLE KVAGEVELCD ISFKYDENML PVLDGLNLHI 

       430        440        450        460        470        480 
KAGETVALVG PSGGGKTTLI KLLLRLYEPS SGSIIIDKID IKDIKLESLR KHVGLVSQDT 

       490        500        510        520        530        540 
TLFSGTIADN IGYRDLTTGI DMKRVELAAK TANADEFIRN LPEGYNTGVG PRGSSLSGGQ 

       550        560        570        580        590        600 
KQRLAIARAL YQKSSILILD EATSALDSLS ELLVREALER VMQDHTVIVI AHRLETVMMA 

       610        620        630 
QRVFLVERGK LKELNRSSLL STHKDSLTSA GLVI 

References and documentation are available.

Number of amino acids: 634

Molecular weight: 69197.86

Theoretical pI: 9.05

Amino acid composition: 
Ala (A)  58	  9.1%
Arg (R)  34	  5.4%
Asn (N)  19	  3.0%
Asp (D)  23	  3.6%
Cys (C)   7	  1.1%
Gln (Q)  20	  3.2%
Glu (E)  37	  5.8%
Gly (G)  34	  5.4%
His (H)   8	  1.3%
Ile (I)  45	  7.1%
Leu (L)  93	 14.7%
Lys (K)  36	  5.7%
Met (M)  11	  1.7%
Phe (F)  21	  3.3%
Pro (P)  27	  4.3%
Ser (S)  64	 10.1%
Thr (T)  30	  4.7%
Trp (W)   2	  0.3%
Tyr (Y)  16	  2.5%
Val (V)  49	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3114
Hydrogen    H	      5103
Nitrogen    N	       829
Oxygen      O	       904
Sulfur      S	        18

Formula: C3114H5103N829O904S18
Total number of atoms: 9968

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35215
Abs 0.1% (=1 g/l)   0.509, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.503, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.94
This classifies the protein as stable.



Aliphatic index: 116.45

Grand average of hydropathicity (GRAVY): 0.231