ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFMDQIPGG GNYPKLPVEC LPNFPIQPSL TFRGRNDSHK LKNFISEIML NMSMISWPND
70 80 90 100 110 120
ASRIVYCRRH LLNPAAQWAN DFVQEQGILE ITFDTFIQGL YQHFYKPPDI NKIFNAITQL
130 140 150 160 170 180
SEAKLGIERL NQRFRKIWDR MPPDFMTEKA AIMTYTRLLT KETYNIVRMH KPETLKDAME
190 200 210 220 230 240
EAYQTTALTE RFFPGFELDA DGDTIIGATT HLQEEYDSDY DSEDNLTQNG YVHTVRTRRS
250 260 270 280 290
YNKPMSNHRN RRNNNPSREE CIKNRLCFYC KKEGHRLNEC RARKASSNRS
References and
documentation are available.
Number of amino acids: 290
Molecular weight: 34026.56
Theoretical pI: 8.65
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1496
Hydrogen H 2324
Nitrogen N 432
Oxygen O 445
Sulfur S 17
Formula: C
1496H
2324N
432O
445S
17
Total number of atoms: 4714
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34755
Abs 0.1% (=1 g/l) 1.021, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 1.010, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.75
This classifies the protein as unstable.
Aliphatic index: 64.97
Grand average of hydropathicity (GRAVY): -0.772