ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH QLRPPSLRIK 

        70         80         90        100        110        120 
NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL QLFSTLKEFY IFQVRYHFFL 

       130        140        150        160        170        180 
HFNNINYLKD IQRWENYYEF PLRYVPIFDV NVNDWALELN SLRHYLLNRN IKFKNNLRTR 

       190        200        210        220        230        240 
LDKLIMDDDF DLADNLIQWL KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM 

       250        260        270        280        290        300 
ETFNKFINQY WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 

       310        320        330        340        350        360 
TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA FLVLDPTGRC 

       370        380        390        400        410        420 
LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF NVDMKALLSL VDTLHDSDIN 

       430        440        450        460        470        480 
QDTNITKRDK NKKSPFLWNL KVKGKRELNK DLPIRHAMLY EHILNYYIAW VPEPNDMIPG 

       490        500        510        520        530        540 
NIKSSYIKTN LFEVLLDLFE SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI 

       550        560        570        580        590        600 
VKFTETSHSN YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 

       610        620        630        640        650        660 
DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP GRKLQLCKDK 

       670        680        690        700        710        720 
GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL SLEQLSESLN IAPPRLTHLL 

       730        740        750        760        770        780 
DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ KTAPMEIENS NYELHNDSEI ERKYELTLQR 

       790        800        810        820        830        840 
SLPFIEGMLT NLGAMKLHKI HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN 

       850 
GSYEIVKNGH KNS 

References and documentation are available.

Number of amino acids: 853

Molecular weight: 99977.89

Theoretical pI: 6.25

Amino acid composition: 
Ala (A)  27	  3.2%
Arg (R)  35	  4.1%
Asn (N)  69	  8.1%
Asp (D)  56	  6.6%
Cys (C)  12	  1.4%
Gln (Q)  28	  3.3%
Glu (E)  54	  6.3%
Gly (G)  22	  2.6%
His (H)  20	  2.3%
Ile (I)  63	  7.4%
Leu (L) 113	 13.2%
Lys (K)  67	  7.9%
Met (M)  18	  2.1%
Phe (F)  47	  5.5%
Pro (P)  31	  3.6%
Ser (S)  56	  6.6%
Thr (T)  50	  5.9%
Trp (W)  13	  1.5%
Tyr (Y)  34	  4.0%
Val (V)  38	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 110
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      4534
Hydrogen    H	      7069
Nitrogen    N	      1175
Oxygen      O	      1311
Sulfur      S	        30

Formula: C4534H7069N1175O1311S30
Total number of atoms: 14119

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   122910
Abs 0.1% (=1 g/l)   1.229, assuming all pairs of Cys residues form cystines


Ext. coefficient   122160
Abs 0.1% (=1 g/l)   1.222, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.04
This classifies the protein as stable.



Aliphatic index: 96.55

Grand average of hydropathicity (GRAVY): -0.334