ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFRDVLERG DEFLEAYPRR SPLWRFLSYS TSLLTFGVSK LLLFTCYNVK LNGFEKLETA 

        70         80         90        100        110        120 
LERSKRENRG LMTVMNHMSM VDDPLVWATL PYKLFTSLDN IRWSLGAHNI CFQNKFLANF 

       130        140        150        160        170        180 
FSLGQVLSTE RFGVGPFQGS IDASIRLLSP DDTLDLEWTP HSEVSSSLKK AYSPPIIRSK 

       190        200        210        220        230        240 
PSWVHVYPEG FVLQLYPPFE NSMRYFKWGI TRMILEATKP PIVVPIFATG FEKIASEAVT 

       250        260        270        280        290        300 
DSMFRQILPR NFGSEINVTI GDPLNDDLID RYRKEWTHLV EKYYDPKNPN DLSDELKYGK 

       310        320        330        340        350        360 
EAQDLRSRLA AELRAHVAEI RNEVRKLPRE DPRFKSPSWW KRFNTTEGKS DPDVKVIGEN 

       370        380 
WAIRRMQKFL PPEGKPKGKD D 

References and documentation are available.

Number of amino acids: 381

Molecular weight: 44187.61

Theoretical pI: 8.80

Amino acid composition: 
Ala (A)  17	  4.5%
Arg (R)  28	  7.3%
Asn (N)  17	  4.5%
Asp (D)  23	  6.0%
Cys (C)   2	  0.5%
Gln (Q)   7	  1.8%
Glu (E)  27	  7.1%
Gly (G)  19	  5.0%
His (H)   6	  1.6%
Ile (I)  18	  4.7%
Leu (L)  40	 10.5%
Lys (K)  26	  6.8%
Met (M)   9	  2.4%
Phe (F)  23	  6.0%
Pro (P)  27	  7.1%
Ser (S)  31	  8.1%
Thr (T)  18	  4.7%
Trp (W)  10	  2.6%
Tyr (Y)  12	  3.1%
Val (V)  21	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2008
Hydrogen    H	      3099
Nitrogen    N	       537
Oxygen      O	       567
Sulfur      S	        11

Formula: C2008H3099N537O567S11
Total number of atoms: 6222

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73005
Abs 0.1% (=1 g/l)   1.652, assuming all pairs of Cys residues form cystines


Ext. coefficient    72880
Abs 0.1% (=1 g/l)   1.649, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.20
This classifies the protein as stable.



Aliphatic index: 79.82

Grand average of hydropathicity (GRAVY): -0.473