ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFRHFKRRL DTSSADESSS ADEEHPDQNV SLTEKSASLS HSDLGGEILN GTGKNRTPND 

        70         80         90        100        110        120 
GQESNESDGS PESDESPESE ESSDNSDSSD SDDMRPLPRP LFMKKKANNL QKATKIDQPW 

       130        140        150        160        170        180 
NAQDDARVLQ TKKENMIKNI DKANQVAKNY ETMKLRLNTN YSTNEELIKQ CLLLDDNDEV 

       190        200        210        220        230        240 
DSEKERQKWF ERQNERKQKH RRIQLAKQRE SEEYEAKRFE AMQKGKDGNT KYDVILDKEK 

       250        260        270        280        290 
EKLDHKKQRS AEKVEKSHNN NRYKITRTKN VEFGDLGKNS RDYEETEYSV I 

References and documentation are available.

Number of amino acids: 291

Molecular weight: 33771.84

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  13	  4.5%
Arg (R)  19	  6.5%
Asn (N)  24	  8.2%
Asp (D)  27	  9.3%
Cys (C)   1	  0.3%
Gln (Q)  15	  5.2%
Glu (E)  34	 11.7%
Gly (G)  10	  3.4%
His (H)   6	  2.1%
Ile (I)   9	  3.1%
Leu (L)  19	  6.5%
Lys (K)  34	 11.7%
Met (M)   6	  2.1%
Phe (F)   6	  2.1%
Pro (P)   8	  2.7%
Ser (S)  30	 10.3%
Thr (T)  13	  4.5%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.4%
Val (V)   8	  2.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1424
Hydrogen    H	      2285
Nitrogen    N	       435
Oxygen      O	       503
Sulfur      S	         7

Formula: C1424H2285N435O503S7
Total number of atoms: 4654

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.635, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.635, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.24
This classifies the protein as unstable.



Aliphatic index: 49.97

Grand average of hydropathicity (GRAVY): -1.538