ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG
70 80 90 100 110 120
SRGGARGGSR GGRGGAAGGA RGGAKVVIEP HRHAGVYIAR GKEDLLVTKN MAPGESVYGE
130 140 150 160 170 180
KRISVEEPSK EDGVPPTKVE YRVWNPFRSK LAAGIMGGLD ELFIAPGKKV LYLGAASGTS
190 200 210 220 230 240
VSHVSDVVGP EGVVYAVEFS HRPGRELISM AKKRPNIIPI IEDARHPQKY RMLIGMVDCV
250 260 270 280 290 300
FADVAQPDQA RIIALNSHMF LKDQGGVVIS IKANCIDSTV DAETVFAREV QKLREERIKP
310 320
LEQLTLEPYE RDHCIVVGRY MRSGLKK
References and
documentation are available.
Number of amino acids: 327
Molecular weight: 34465.28
Theoretical pI: 10.24
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 1494
Hydrogen H 2426
Nitrogen N 476
Oxygen O 441
Sulfur S 11
Formula: C
1494H
2426N
476O
441S
11
Total number of atoms: 4848
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17545
Abs 0.1% (=1 g/l) 0.509, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.505, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.01
This classifies the protein as stable.
Aliphatic index: 72.14
Grand average of hydropathicity (GRAVY): -0.412