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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG

70 80 90 100 110 120
SRGGARGGSR GGRGGAAGGA RGGAKVVIEP HRHAGVYIAR GKEDLLVTKN MAPGESVYGE

130 140 150 160 170 180
KRISVEEPSK EDGVPPTKVE YRVWNPFRSK LAAGIMGGLD ELFIAPGKKV LYLGAASGTS

190 200 210 220 230 240
VSHVSDVVGP EGVVYAVEFS HRPGRELISM AKKRPNIIPI IEDARHPQKY RMLIGMVDCV

250 260 270 280 290 300
FADVAQPDQA RIIALNSHMF LKDQGGVVIS IKANCIDSTV DAETVFAREV QKLREERIKP

310 320
LEQLTLEPYE RDHCIVVGRY MRSGLKK


References and documentation are available.

Number of amino acids: 327 Molecular weight: 34465.28 Theoretical pI: 10.24
Amino acid composition: 
Ala (A) 25 7.6% Arg (R) 35 10.7% Asn (N) 5 1.5% Asp (D) 12 3.7% Cys (C) 3 0.9% Gln (Q) 6 1.8% Glu (E) 20 6.1% Gly (G) 62 19.0% His (H) 7 2.1% Ile (I) 18 5.5% Leu (L) 16 4.9% Lys (K) 18 5.5% Met (M) 8 2.4% Phe (F) 10 3.1% Pro (P) 16 4.9% Ser (S) 24 7.3% Thr (T) 6 1.8% Trp (W) 1 0.3% Tyr (Y) 8 2.4% Val (V) 27 8.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 32 Total number of positively charged residues (Arg + Lys): 53 Atomic composition: Carbon C 1494 Hydrogen H 2426 Nitrogen N 476 Oxygen O 441 Sulfur S 11 Formula: C1494H2426N476O441S11 Total number of atoms: 4848 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17545 Abs 0.1% (=1 g/l) 0.509, assuming all pairs of Cys residues form cystines Ext. coefficient 17420 Abs 0.1% (=1 g/l) 0.505, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.01 This classifies the protein as stable. Aliphatic index: 72.14 Grand average of hydropathicity (GRAVY): -0.412