ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFSKAKLKR FNDVDVAICG SPAASNSSAG SAGSATPTAS SAAAAPPTVQ PERKEQIEKF 

        70         80         90        100        110        120 
FKDAVRFASS SKEAKEFAIP KEDKKSKGLR LFRTPSLPQR LRFRPTPSHT DTATGSGSGA 

       130        140        150        160        170        180 
STAASTPLHS AATTPVKEAK SASRLKGKEA LQYEIRHKNE LIESQLSQLD VLRRHVDQLK 

       190        200        210        220        230        240 
EAEAKLREEH ELATSKTDRL IEALTSENLS HKALNEQMGQ EHADLLERLA AMEQQLQQQH 

       250        260        270        280        290        300 
DEHERQVEAL VAESEALRLA NELLQTANED RQKVEEQLQA QLSALQADVA QAREHCSLEQ 

       310        320        330        340        350        360 
AKTAENIELV ENLQKTNASL LADVVQLKQQ IEQDALSYGQ EAKSCQAELE CLKVERNTLK 

       370        380        390        400        410        420 
NDLANKCTLI RSLQDELLDK NCEIDAHCDT IRQLCREQAR HTEQQQAVAK VQQQVESDLE 

       430        440        450        460        470        480 
SAVEREKSYW RAELDKRQKL AENELIKIEL EKQDVMVLLE TTNDMLRMRD EKLQKCEEQL 

       490        500        510        520        530        540 
RNGIDYYIQL SDALQQQLVQ LKQDMAKTIT EKYNYQLTLT NTRATVNILM ERLKKSDADV 

       550        560        570        580        590        600 
EQYRAELESV QLAKGALEQS YLVLQADAEQ LRQQLTESQD ALNALRSSSQ TLQSEVSLKE 

       610        620        630        640        650        660 
SLLHELLAGE AETLAKFNQI ANSFQERIDG DAQLAHYHEL RRKDETREAY MVDMKKALDE 

       670        680        690        700        710        720 
FATVLQFAQL ELDNKEQMLV KVREECEQLK LENIALKSKQ PGSASLLGTP GKANRSNTTD 

       730        740        750        760        770        780 
LEKIEDLLCD SELRSDCEKI TTWLLNSSDK CVRQDTTSEI NELLSAGKSS PRPAPRTPKA 

       790        800        810        820 
PHTPRSPRTP HTPRTPRSAA STPKKTVLFA GKENVPSPPQ KQVLKARNI 

References and documentation are available.

Number of amino acids: 829

Molecular weight: 93100.74

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  89	 10.7%
Arg (R)  50	  6.0%
Asn (N)  31	  3.7%
Asp (D)  43	  5.2%
Cys (C)  13	  1.6%
Gln (Q)  71	  8.6%
Glu (E)  89	 10.7%
Gly (G)  19	  2.3%
His (H)  17	  2.1%
Ile (I)  24	  2.9%
Leu (L) 103	 12.4%
Lys (K)  65	  7.8%
Met (M)  11	  1.3%
Phe (F)  13	  1.6%
Pro (P)  28	  3.4%
Ser (S)  66	  8.0%
Thr (T)  49	  5.9%
Trp (W)   2	  0.2%
Tyr (Y)  11	  1.3%
Val (V)  35	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 132
Total number of positively charged residues (Arg + Lys): 115

Atomic composition:

Carbon      C	      3996
Hydrogen    H	      6576
Nitrogen    N	      1182
Oxygen      O	      1322
Sulfur      S	        24

Formula: C3996H6576N1182O1322S24
Total number of atoms: 13100

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28140
Abs 0.1% (=1 g/l)   0.302, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.294, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.40
This classifies the protein as unstable.



Aliphatic index: 82.73

Grand average of hydropathicity (GRAVY): -0.737