ProtParam
User-provided sequence:
10 20 30 40 50 60
MSFSVEKKTT LAHPFRAPGS TLISSDQSNL KIVYNKYKTR SPLPNSTNQL RYNFIFCHGT
70 80 90 100 110 120
GFNKSIWKYH ISKLYQLSQS LQVPWFLDSV IAIDMVGHGD SSLENQGKLG TIFRWDDGAK
130 140 150 160 170 180
DVIAIIKHEI ATTGDFQNNL ESRNLIIGHS MGGFNSLYAT FLEPSLFDAV IPIEAVIYGA
190 200 210 220 230 240
PGGLEKFSKK FSKISKLLID TFDSKDDINF FFKEFSFFKN MQDQVSDDFI NDEVYEIKDK
250 260 270 280 290 300
ESGEIKYKLK CNTPHQMAAY YGAFMSIPFG MFAIPHIRVP ICHVIGSKGV WNPPESITWI
310 320 330 340 350 360
RGAINKEFLA GKVDVPKGEH LLNVELPDET IDIIQNFTTE RTKAFIEARN NLPEVKLNNN
370 380 390 400
KEAIAKEQFQ NLIDLKFDQV NGYFIEDRVD NYEALQKLAK L
References and
documentation are available.
Number of amino acids: 401
Molecular weight: 45631.10
Theoretical pI: 6.23
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2082
Hydrogen H 3197
Nitrogen N 533
Oxygen O 601
Sulfur S 10
Formula: C
2082H
3197N
533O
601S
10
Total number of atoms: 6423
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46995
Abs 0.1% (=1 g/l) 1.030, assuming all pairs of Cys residues form cystines
Ext. coefficient 46870
Abs 0.1% (=1 g/l) 1.027, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.87
This classifies the protein as stable.
Aliphatic index: 86.33
Grand average of hydropathicity (GRAVY): -0.280