ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFVFFILLT FYIQVKGSVD EYRLLQYLKE NYDSFERPVE NSSAPLDVKV RFLLNQIMDV 

        70         80         90        100        110        120 
DEKNQVMSIL AYMDYHWNDY KLTWDPKLFG GIRDVRFSGD DDASFKLWRP DVLLFNSVSE 

       130        140        150        160        170        180 
SFDSTYSSRF IVSSNGDVQQ NPPGIFRFVC QIDVTYYPFD KQTCFLKLGS WTYNGQYINL 

       190        200        210        220        230        240 
DFLLTQIIGL HGIPIVVEPE RSSTNYASYF VNESIDLQVY LQNGEWDLEG TPGKRVVQKF 

       250        260        270        280        290        300 
GTDEYHELYF YIHIRRRTLA YGINLIIPSL VISMMTILGF TLPPDACEKI TLETTVLLSV 

       310        320        330        340        350        360 
IFFLQMVSST SPPQSQSVPI LAAFFSCCLM LVACSCVFTV LTLSLHHRKP ETHEMSPTMR 

       370        380        390        400        410        420 
KIFIDWLPYI LCMCKPDHPK TPKPKVLGKS IAPIHRLSTI PMLNLPRVSQ TTFPDKNVFV 

       430        440        450        460        470        480 
YHEVPAVIKN TSRICAELLI IERDIEKMLD KIEEDTKEDM LRSEWRFAAM AVDRLCLYVF 

       490        500 
SAFITMITCG LIVPHILANI 

References and documentation are available.

Number of amino acids: 500

Molecular weight: 57675.05

Theoretical pI: 5.72

Amino acid composition: 
Ala (A)  17	  3.4%
Arg (R)  22	  4.4%
Asn (N)  18	  3.6%
Asp (D)  30	  6.0%
Cys (C)  12	  2.4%
Gln (Q)  17	  3.4%
Glu (E)  25	  5.0%
Gly (G)  18	  3.6%
His (H)  11	  2.2%
Ile (I)  41	  8.2%
Leu (L)  53	 10.6%
Lys (K)  24	  4.8%
Met (M)  16	  3.2%
Phe (F)  32	  6.4%
Pro (P)  29	  5.8%
Ser (S)  39	  7.8%
Thr (T)  29	  5.8%
Trp (W)   7	  1.4%
Tyr (Y)  22	  4.4%
Val (V)  38	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2642
Hydrogen    H	      4076
Nitrogen    N	       654
Oxygen      O	       736
Sulfur      S	        28

Formula: C2642H4076N654O736S28
Total number of atoms: 8136

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72030
Abs 0.1% (=1 g/l)   1.249, assuming all pairs of Cys residues form cystines


Ext. coefficient    71280
Abs 0.1% (=1 g/l)   1.236, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.42
This classifies the protein as unstable.



Aliphatic index: 98.76

Grand average of hydropathicity (GRAVY): 0.087