ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFWPFGQNL NHSNINKILD EYFHVLHELE RINPSVGKAI PAIFNNVQER GTSDSLDSIP 

        70         80         90        100        110        120 
EEYSHGDEVK TARGDQKSRF EKDDQQERYE KEEEERSMNS SESSTTSFSS GSTSKTDLDE 

       130        140        150        160        170        180 
EDISNATAPM MVTTKNLDNS FIERMLVETE LLNELSRQNK TLLDFICFGF FFDKKTNKKV 

       190        200        210        220        230        240 
NNMEYLVDQL MECISKIKTA TTVDLNNLID YQEQQQLDDS SQEDVYVESD TEQEEEKEDD 

       250        260        270        280        290        300 
NNSNNKKRRK RGSSSFGNDD INNNDDDDDA NEDDESAYLT KATIISEIFS LDIWLISESL 

       310        320        330        340        350        360 
VKNQSYLNKI WSIINQPNFN SENSPLVPIF LKINQNLLLT RQDQYLNFIR TERSFVDDML 

       370        380        390        400        410        420 
KHVDISLLMD FFLKIISTDK IESPTGIIEL VYDQNLISKC LSFLNNKESP ADIQACVGDF 

       430        440        450        460        470        480 
LKALIAISAN APLDDISIGP NSLTRQLASP ESIAKLVDIM INQRGAALNT TVSIVIELIR 

       490        500        510        520        530        540 
KNNSDYDQVN LLTTTIKTHP PSNRDPIYLG YLLRKFSNHL SDFFQIILDI ENDANIPLHE 

       550        560        570        580        590        600 
NQLHEKFKPL GFERFKVVEL IAELLHCSNM GLMNSKRAER IARRRDKVRS QLSHHLQDAL 

       610        620        630        640        650        660 
NDLSIEEKEQ LKTKHSPTRD TDHDLKNNNG KIDNDNNDND DESDYGDEID ESFEIPYINM 

       670        680        690        700        710        720 
KQTIKLRTDP TVGDLFKIKL YDTRIVSKIM ELFLTHPWNN FWHNVIFDII QQIFNGRMDF 

       730        740        750        760        770        780 
SYNSFLVLSL FNLKSSYQFM TDIVISDEKG TDVSRFSPVI RDPNFDFKIT TDFILRGYQD 

       790        800        810        820        830        840 
SYKFYELRKM NLGYMGHIVL IAEEVVKFSK LYKVELISPD IQVILQTEEW QYYSEEVLNE 

       850        860        870        880        890        900 
TRMMYSKILG GGSYIDDGNG NIIPQLPDNT TVLTPNGDAS NNNEILDSDT GSSNGTSGGG 

       910        920        930        940        950        960 
QLINVESLEE QLSLSTESDL HNKLREMLIN RAQEDVDNKN TENGVFILGP PEDKNSNSNI 

       970        980        990       1000 
NNTNHNSNNS NNNDNNDNND NDNDNTRNYN EDADNDNDYD HE 

References and documentation are available.

Number of amino acids: 1002

Molecular weight: 114989.22

Theoretical pI: 4.55

Amino acid composition: 
Ala (A)  28	  2.8%
Arg (R)  38	  3.8%
Asn (N) 108	 10.8%
Asp (D)  97	  9.7%
Cys (C)   5	  0.5%
Gln (Q)  40	  4.0%
Glu (E)  78	  7.8%
Gly (G)  37	  3.7%
His (H)  20	  2.0%
Ile (I)  82	  8.2%
Leu (L)  94	  9.4%
Lys (K)  60	  6.0%
Met (M)  21	  2.1%
Phe (F)  45	  4.5%
Pro (P)  30	  3.0%
Ser (S)  91	  9.1%
Thr (T)  52	  5.2%
Trp (W)   6	  0.6%
Tyr (Y)  29	  2.9%
Val (V)  41	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 175
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      5020
Hydrogen    H	      7842
Nitrogen    N	      1370
Oxygen      O	      1673
Sulfur      S	        26

Formula: C5020H7842N1370O1673S26
Total number of atoms: 15931

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76460
Abs 0.1% (=1 g/l)   0.665, assuming all pairs of Cys residues form cystines


Ext. coefficient    76210
Abs 0.1% (=1 g/l)   0.663, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.54
This classifies the protein as unstable.



Aliphatic index: 83.16

Grand average of hydropathicity (GRAVY): -0.686