ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSFYQPSLSL YDVLNALSNQ TGQRGQQGYP RQPQRPQRYH PHYGQVHVGG HHPRHHPLYS 

        70         80         90        100        110        120 
RYNGVPNTYY YQFPGQAYYY SPEYGYDDED GEEEDQDEDM VGDSGTTRQE DGGEDSNSRR 

       130        140        150        160        170        180 
YPSYYHCNTA RNNRTNQQAN SLNDLLTALI GVPPYEGTEP EIEANTEQEG EKGEEKDKKD 

       190        200        210        220        230        240 
KSEAPKEEAG ETNKEKPLNQ LEESSRPPLA KKSSSFAHLQ APSPIPDPLQ VSKPETRMDL 

       250        260        270        280        290        300 
PFSPEVNVYD TEDTYVVVLA LPGANSRAFH IDYHPSSHEM LIKGKIEDRV GIDEKFLKIT 

       310        320        330        340        350        360 
ELKYGAFERT VKFPVLPRIK DEEIKATYNN GLLQIKVPKI VNDTEKPKPK KRIAIEEIPD 

       370 
EELEFEENPN PTVEN 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 42817.02

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  17	  4.5%
Arg (R)  18	  4.8%
Asn (N)  22	  5.9%
Asp (D)  23	  6.1%
Cys (C)   1	  0.3%
Gln (Q)  20	  5.3%
Glu (E)  43	 11.5%
Gly (G)  24	  6.4%
His (H)  12	  3.2%
Ile (I)  15	  4.0%
Leu (L)  24	  6.4%
Lys (K)  25	  6.7%
Met (M)   4	  1.1%
Phe (F)   9	  2.4%
Pro (P)  35	  9.3%
Ser (S)  23	  6.1%
Thr (T)  18	  4.8%
Trp (W)   0	  0.0%
Tyr (Y)  24	  6.4%
Val (V)  18	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1884
Hydrogen    H	      2882
Nitrogen    N	       520
Oxygen      O	       615
Sulfur      S	         5

Formula: C1884H2882N520O615S5
Total number of atoms: 5906

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35760
Abs 0.1% (=1 g/l)   0.835, assuming all pairs of Cys residues form cystines


Ext. coefficient    35760
Abs 0.1% (=1 g/l)   0.835, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.56
This classifies the protein as unstable.



Aliphatic index: 59.01

Grand average of hydropathicity (GRAVY): -1.127