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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGAAKRVAT SSVNWSKLAE RLVPEHAAEL TRVKGVSGTF QSAVSQLPAD LPKIDFAALK

70 80 90 100 110 120
KALPAHSAVL DSLQKQYESV KIPYGEVPAE YLKEVDQWVD YNNARIKLHE VKVADGLQEA

130 140 150 160 170 180
KKVEEKWAKA PPVEHFDRQH FVEYFPAHFY DLRYQNRIPD PCNIGLNETP EIENRFKDYK

190
VLRRADKVDD H


References and documentation are available.

Number of amino acids: 191 Molecular weight: 21797.70 Theoretical pI: 6.66
Amino acid composition: 
Ala (A) 21 11.0% Arg (R) 10 5.2% Asn (N) 7 3.7% Asp (D) 13 6.8% Cys (C) 1 0.5% Gln (Q) 8 4.2% Glu (E) 16 8.4% Gly (G) 6 3.1% His (H) 7 3.7% Ile (I) 6 3.1% Leu (L) 15 7.9% Lys (K) 18 9.4% Met (M) 1 0.5% Phe (F) 7 3.7% Pro (P) 12 6.3% Ser (S) 10 5.2% Thr (T) 4 2.1% Trp (W) 3 1.6% Tyr (Y) 8 4.2% Val (V) 18 9.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 29 Total number of positively charged residues (Arg + Lys): 28 Atomic composition: Carbon C 983 Hydrogen H 1527 Nitrogen N 271 Oxygen O 287 Sulfur S 2 Formula: C983H1527N271O287S2 Total number of atoms: 3070 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 28420 Abs 0.1% (=1 g/l) 1.304, assuming all pairs of Cys residues form cystines Ext. coefficient 28420 Abs 0.1% (=1 g/l) 1.304, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.87 This classifies the protein as stable. Aliphatic index: 81.20 Grand average of hydropathicity (GRAVY): -0.606