ProtParam
User-provided sequence:
10 20 30 40 50 60
MSGAAKRVAT SSVNWSKLAE RLVPEHAAEL TRVKGVSGTF QSAVSQLPAD LPKIDFAALK
70 80 90 100 110 120
KALPAHSAVL DSLQKQYESV KIPYGEVPAE YLKEVDQWVD YNNARIKLHE VKVADGLQEA
130 140 150 160 170 180
KKVEEKWAKA PPVEHFDRQH FVEYFPAHFY DLRYQNRIPD PCNIGLNETP EIENRFKDYK
190
VLRRADKVDD H
References and
documentation are available.
Number of amino acids: 191
Molecular weight: 21797.70
Theoretical pI: 6.66
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 983
Hydrogen H 1527
Nitrogen N 271
Oxygen O 287
Sulfur S 2
Formula: C
983H
1527N
271O
287S
2
Total number of atoms: 3070
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 1.304, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 1.304, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.87
This classifies the protein as stable.
Aliphatic index: 81.20
Grand average of hydropathicity (GRAVY): -0.606