ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGAGEALPP GPVGQQCPAE AGGGRLGSPA HEECNEDTTE KEKRQTTSSH TRCGLIRDQS 

        70         80         90        100        110        120 
IWRNPSDGEL VRTQPERLPQ LQTSAQEPSK EETSKIQNGG HTTMNNGNGI PHRAKHMSMD 

       130        140        150        160        170        180 
NHKMSSPVSQ KMHRKIQSSL SVNNDVNKKS KVNAVFSQKS ASSPEDCCVH CILACLFCEF 

       190        200        210        220        230        240 
LTLCNIVLGQ ASCGVCTSEA CCCCCGDEMG DDCSCPCDMD CSIMDACCES SDCLEICMEC 


CGICFPS 

References and documentation are available.

Number of amino acids: 247

Molecular weight: 26527.89

Theoretical pI: 5.16

Amino acid composition: 
Ala (A)  13	  5.3%
Arg (R)   9	  3.6%
Asn (N)  12	  4.9%
Asp (D)  13	  5.3%
Cys (C)  27	 10.9%
Gln (Q)  13	  5.3%
Glu (E)  19	  7.7%
Gly (G)  19	  7.7%
His (H)   8	  3.2%
Ile (I)  10	  4.0%
Leu (L)  13	  5.3%
Lys (K)  12	  4.9%
Met (M)  10	  4.0%
Phe (F)   4	  1.6%
Pro (P)  14	  5.7%
Ser (S)  28	 11.3%
Thr (T)  12	  4.9%
Trp (W)   1	  0.4%
Tyr (Y)   0	  0.0%
Val (V)  10	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1079
Hydrogen    H	      1742
Nitrogen    N	       328
Oxygen      O	       377
Sulfur      S	        37

Formula: C1079H1742N328O377S37
Total number of atoms: 3563

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7125
Abs 0.1% (=1 g/l)   0.269, assuming all pairs of Cys residues form cystines


Ext. coefficient     5500
Abs 0.1% (=1 g/l)   0.207, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 75.23
This classifies the protein as unstable.



Aliphatic index: 53.32

Grand average of hydropathicity (GRAVY): -0.472