ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD IANRVEAMKK 

        70         80         90        100        110        120 
LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL GKFVPYIGGP QYATILLPVL 

       130        140        150        160        170        180 
EILASAEETL VREKAVDSLN NVAQELSQEQ LFSDFVPLIE HLATADWFSS KVSACGLFKS 

       190        200        210        220        230        240 
VIVRIKDDSL RKNILALYLQ LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS 

       250        260        270        280        290        300 
NIFQKIINDN QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 

       310        320        330        340        350        360 
ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF LNDPSIILNK 

       370        380        390        400        410        420 
ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF LPILLNMLRD EFPDVRLNII 

       430        440        450        460        470        480 
ASLKVVNDVI GIELLSDSLL PAITELAKDV NWRVRMAIIE YIPILAEQLG MQFFDQQLSD 

       490        500        510        520        530        540 
LCLSWLWDTV YSIREAAVNN LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS 

       550        560        570        580        590        600 
ALTTLVPVVS LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 

       610        620        630 
NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN 

References and documentation are available.

Number of amino acids: 635

Molecular weight: 70907.39

Theoretical pI: 4.55

Amino acid composition: 
Ala (A)  53	  8.3%
Arg (R)  23	  3.6%
Asn (N)  35	  5.5%
Asp (D)  53	  8.3%
Cys (C)   7	  1.1%
Gln (Q)  27	  4.3%
Glu (E)  39	  6.1%
Gly (G)  17	  2.7%
His (H)   4	  0.6%
Ile (I)  47	  7.4%
Leu (L)  91	 14.3%
Lys (K)  35	  5.5%
Met (M)   9	  1.4%
Phe (F)  27	  4.3%
Pro (P)  21	  3.3%
Ser (S)  50	  7.9%
Thr (T)  29	  4.6%
Trp (W)   7	  1.1%
Tyr (Y)  12	  1.9%
Val (V)  49	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      3185
Hydrogen    H	      5102
Nitrogen    N	       816
Oxygen      O	       973
Sulfur      S	        16

Formula: C3185H5102N816O973S16
Total number of atoms: 10092

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56755
Abs 0.1% (=1 g/l)   0.800, assuming all pairs of Cys residues form cystines


Ext. coefficient    56380
Abs 0.1% (=1 g/l)   0.795, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.95
This classifies the protein as unstable.



Aliphatic index: 115.48

Grand average of hydropathicity (GRAVY): 0.086