ProtParam
User-provided sequence:
10 20 30 40 50 60
MSGDNGGGER RKGSVKWFDT QKGFGFITPD DGGDDLFVHQ SSIRSEGFRS LAAEEAVEFE
70 80 90 100 110 120
VEIDNNNRPK AIDVSGPDGA PVQGNSGGGS SGGRGGFGGG RGGGRGSGGG YGGGGGGYGG
130 140 150 160 170 180
RGGGGRGGSD CYKCGEPGHM ARDCSEGGGG YGGGGGGYGG GGGYGGGGGG YGGGGRGGGG
190 200
GGGSCYSCGE SGHFARDCTS GGR
References and
documentation are available.
Number of amino acids: 203
Molecular weight: 19153.10
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 787
Hydrogen H 1179
Nitrogen N 265
Oxygen O 284
Sulfur S 8
Formula: C
787H
1179N
265O
284S
8
Total number of atoms: 2523
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17795
Abs 0.1% (=1 g/l) 0.929, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.910, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.39
This classifies the protein as unstable.
Aliphatic index: 23.55
Grand average of hydropathicity (GRAVY): -0.790