ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG VPSTAIREIS 

        70         80         90        100        110        120 
LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME GIPKDQPLGA DIVKKFMMQL 

       130        140        150        160        170        180 
CKGIAYCHSH RILHRDLKPQ NLLINKDGNL KLGDFGLARA FGVPLRAYTH EIVTLWYRAP 

       190        200        210        220        230        240 
EVLLGGKQYS TGVDTWSIGC IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV 

       250        260        270        280        290 
YLPDFKPSFP QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES 

References and documentation are available.

Number of amino acids: 298

Molecular weight: 34061.50

Theoretical pI: 8.50

Amino acid composition: 
Ala (A)  17	  5.7%
Arg (R)  20	  6.7%
Asn (N)   8	  2.7%
Asp (D)  23	  7.7%
Cys (C)   4	  1.3%
Gln (Q)  10	  3.4%
Glu (E)  16	  5.4%
Gly (G)  21	  7.0%
His (H)   7	  2.3%
Ile (I)  23	  7.7%
Leu (L)  36	 12.1%
Lys (K)  22	  7.4%
Met (M)   5	  1.7%
Phe (F)  12	  4.0%
Pro (P)  17	  5.7%
Ser (S)  15	  5.0%
Thr (T)   7	  2.3%
Trp (W)   4	  1.3%
Tyr (Y)  13	  4.4%
Val (V)  18	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1549
Hydrogen    H	      2442
Nitrogen    N	       416
Oxygen      O	       430
Sulfur      S	         9

Formula: C1549H2442N416O430S9
Total number of atoms: 4846

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41620
Abs 0.1% (=1 g/l)   1.222, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   1.215, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.65
This classifies the protein as stable.



Aliphatic index: 100.44

Grand average of hydropathicity (GRAVY): -0.239