ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGFIKSTLL GLGQDYLEDQ YQEFAEQHFQ PTRDPFYETN KDGKKHRRRL PYYCTKDESK 

        70         80         90        100        110        120 
AWKKVQNKAW LHDKSLCGCC CWTNTIGWAP LLALLPVIGP LLMYWVHDKL IELADDRYKL 

       130        140        150        160        170        180 
PAEIKVKMHG NIVIDLLISL VPILGSVFAW LHACSTRNAA IVYNFVGKRA LERKQAELMH 

       190        200        210        220        230        240 
QKEENEKHSN ANTAPPVVGG NKNVNGNRNN SKMYNRPPVT APPAPAYTRS TNGRPQRGYR 

References and documentation are available.

Number of amino acids: 240

Molecular weight: 27423.52

Theoretical pI: 9.43

Amino acid composition: 
Ala (A)  18	  7.5%
Arg (R)  14	  5.8%
Asn (N)  17	  7.1%
Asp (D)  10	  4.2%
Cys (C)   6	  2.5%
Gln (Q)   9	  3.8%
Glu (E)  12	  5.0%
Gly (G)  15	  6.2%
His (H)   8	  3.3%
Ile (I)  10	  4.2%
Leu (L)  23	  9.6%
Lys (K)  20	  8.3%
Met (M)   5	  2.1%
Phe (F)   6	  2.5%
Pro (P)  16	  6.7%
Ser (S)  10	  4.2%
Thr (T)  11	  4.6%
Trp (W)   6	  2.5%
Tyr (Y)  11	  4.6%
Val (V)  13	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1228
Hydrogen    H	      1916
Nitrogen    N	       350
Oxygen      O	       343
Sulfur      S	        11

Formula: C1228H1916N350O343S11
Total number of atoms: 3848

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49765
Abs 0.1% (=1 g/l)   1.815, assuming all pairs of Cys residues form cystines


Ext. coefficient    49390
Abs 0.1% (=1 g/l)   1.801, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.04
This classifies the protein as unstable.



Aliphatic index: 76.83

Grand average of hydropathicity (GRAVY): -0.587