ProtParam
User-provided sequence:
10 20 30 40 50 60
MSGFRAFKAQ VPIEWSQSLY ITLVRGLPGT RKLHRRTLEA MGLRRCHRTV LHSNNSSIRG
70 80 90 100
MIDQVKRMVV VETEEMYNAR KEAEANHKAL RPPLVVSHSS PATDSSNMS
References and
documentation are available.
Number of amino acids: 109
Molecular weight: 12359.26
Theoretical pI: 10.59
Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 17
Atomic composition:
Carbon C 532
Hydrogen H 875
Nitrogen N 169
Oxygen O 156
Sulfur S 7
Formula: C
532H
875N
169O
156S
7
Total number of atoms: 1739
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.686, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.686, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 69.34
This classifies the protein as unstable.
Aliphatic index: 77.80
Grand average of hydropathicity (GRAVY): -0.481