ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGFSLSEYL TKFQTTDRES YPRLQDPSRE LNVIIDQLAV SPEQIDASPD SLEALIDLCH 

        70         80         90        100        110        120 
DFPHLTPKLQ TQLSYLISSS LSNLSKDIKA NLSSNVNFTE IGGLIPQWKR HLEEYGYLIQ 

       130        140        150        160        170        180 
VLLTFLQDEL HKVSSQSTNL NRSAKNSKND SANVELFKRD CNQMENLLES ITKLLEINLS 

       190        200        210        220        230        240 
KIFQTTPEKD LFIGLFTRPL FVLLEIEPVT KVSSLKMFIQ RILAMCVKNH GQSSSIQSSL 

       250        260        270        280        290        300 
MTNLTYFLHL SVFNAELLKL LNDEYNYPQL TEDILKEIST RVFNAKDTTG PKAISNFLIK 

       310        320        330        340        350        360 
LSELSPGIML RQMNLVITLL NNSSITLRCS VVEACGNIVA ELAQDPQTME HYKQQIAVLI 

       370        380        390        400        410        420 
ELLEERFQDS NPYVRTKAIQ GCSKICDLSS KFNKSKAKFT SLAVRSLQDR SSLVRRNSVK 

       430        440        450        460        470        480 
LLSKLLLKHP FKAIHGSQLR LSEWEEYLKG SESQLNSTLK KVESQETLND TIERSLIEEE 

       490        500        510        520        530        540 
VEQDEGQCRT ELEGSFNKSA ELSRIENEVE NINATNTSVL MKLKLMIVYY KDAISFIKEI 

       550        560        570        580        590        600 
HKSIELISNL LFSKNRNEVL ESMDFLVLAD AFDIELSEFG IKKMLHLVWM KGTNDEGTSI 

       610        620        630        640        650        660 
SVHLIECYKQ LFLTAPDSCN MQEKAAHIAK NLINLSIGAS IADLASLEQL LGMMYEQKLI 

       670        680        690        700        710        720 
DQHVINILWA IYNSASKASM QKEQNVNNRD SEKGFSKEQI HGSIIILGML SLADNEIALK 

       730        740        750        760        770        780 
GLESLLNIGL GAVGLKDLTL CRYSCLALER MVPKRSTIIT KAINQELEDV AVKKLYAIII 

       790        800        810        820        830        840 
NYTKDNEYYP MCEQALSALF TISSKPDILA TDLIREKTMM TFGKPEEEDS ILSLEQSSRV 

       850        860        870        880        890        900 
VSLSQLLFIV GQVAIKTLVY LEKCEAEFKK RKIEAETRNG KVKNQGADVT NTTQDNGGDK 

       910        920        930        940        950        960 
ELEMIGGTNE DDFTDAIQFV KENELLFGEK SILGKFCPIV EEIVSNSSRF SDPMLQRTAT 

       970        980        990       1000       1010       1020 
LCLEKLMCLS SKYCEKSLPL LITVMEKSPD PTIRSNAVLG LGDMAVCFNN LVDENTDYLY 

      1030       1040       1050       1060       1070       1080 
RRLHDENLMV QRTCLMTVTF LILAGQVKVK GQLGEMAKCL DNPDQGISDM CRLFFTELAS 

      1090       1100       1110       1120       1130       1140 
KDNAIYNGFI DIFSNLSSDD LLGKESFKKI IKFLLTFIDK ERHQKQLNEK LVGRLRKCET 

      1150       1160       1170 
QKQWDDIAFV LNNLPYKNED VTALLEQGFK VVSAKE 

References and documentation are available.

Number of amino acids: 1176

Molecular weight: 132967.15

Theoretical pI: 5.43

Amino acid composition: 
Ala (A)  58	  4.9%
Arg (R)  40	  3.4%
Asn (N)  71	  6.0%
Asp (D)  60	  5.1%
Cys (C)  23	  2.0%
Gln (Q)  55	  4.7%
Glu (E)  98	  8.3%
Gly (G)  46	  3.9%
His (H)  17	  1.4%
Ile (I)  87	  7.4%
Leu (L) 162	 13.8%
Lys (K)  93	  7.9%
Met (M)  31	  2.6%
Phe (F)  47	  4.0%
Pro (P)  28	  2.4%
Ser (S) 109	  9.3%
Thr (T)  60	  5.1%
Trp (W)   5	  0.4%
Tyr (Y)  27	  2.3%
Val (V)  59	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 158
Total number of positively charged residues (Arg + Lys): 133

Atomic composition:

Carbon      C	      5896
Hydrogen    H	      9538
Nitrogen    N	      1554
Oxygen      O	      1815
Sulfur      S	        54

Formula: C5896H9538N1554O1815S54
Total number of atoms: 18857

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69105
Abs 0.1% (=1 g/l)   0.520, assuming all pairs of Cys residues form cystines


Ext. coefficient    67730
Abs 0.1% (=1 g/l)   0.509, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.21
This classifies the protein as unstable.



Aliphatic index: 102.06

Grand average of hydropathicity (GRAVY): -0.184